摘要
选用葛根素作为内标物质,利用内标标准曲线法建立高效液相色谱同时测定茶叶中(-)表没食子儿茶素(EGC),(-)表没食子儿茶素没食子酸酯(EGCG),(-)表儿茶素(EC),(-)表儿茶素没食子酸酯(ECG),咖啡因(CAF)5种组分的分析方法.采用C18柱,甲醇和0.05%三氟乙酸水溶液为流动相,梯度洗脱,DAD检测器,检测波长278 nm.结果表明,各组分的色谱峰均达到基线分离,该方法重复性好,灵敏度高,回收率高,已用于不同种类26个茶叶样品的测定.以这5种活性成分为指标,采用聚类分析法可对不同来源的样品进行统计分析.
An internal standard curve method with puerarin as an internal standard was established for measuring five components in tea simultaneously by HPLC,they are epigallocatechin（EGC）,epigallocatechin gallate（EGCG）,epicatechin（EC） and epicaechin gallate（ECG）and caffeine.The tea extract was separated using a C18 column（4.5×250mm,5um） with methanol-0.05% trifluoroacetic acid as the eluent in gradient elution mode.All components were baseline separated peaks at 278nm accurately.This reproducibility,high sensitivity and high recovery rate are acceptable for determining the catechins and caffeine in 26 tea samples with satisfactory results.The cluster analysis has been used to discuss the results of tea samples from different origins.
出处
《昆明学院学报》
2010年第3期47-50,共4页
Journal of Kunming University
基金
云南大学骨干教师培训计划资助项目(W33020000)
