摘要
以野生型拟南芥（Ａｒａｂｉｄｏｐｓｉｓｔｈａｌｉａｎａ（Ｌ．）Ｈｅｙｎｈ．）为材料，克隆并测序了Ｌｅａｆｙ（ＬＦＹ）基因的全长ｃＤＮＡ；同时构建了ＬＦＹｃＤＮＡ以ＣａＭＶ３５Ｓ为启动子的植物表达载体，并转化菊花（Ｃｈｒｙｓａｎｔｈｅｍｕｍｍｏｒｉｆｏｌｉｕｍ（Ｒａｍａｔ．）Ｔｚｖｅｌ．）。该ｃＤＮＡ全长１２６３ｂｐ，共编码４２０个氨基酸残基，Ｓｏｕｔｈｅｒｎ杂交结果表明，ＬＦＹｃＤＮＡ整合到菊花染色体组中。跟正常植株相比，转基因植株中有３株分别提早６５、６７、７０ｄ开花，２株分别推迟７８、９０ｄ开花。
The total length cDNA of Leafy (LFY) was cloned and sequenced from wild type Arabidopsis thaliana (L.) Heynh. The plant expression vector with CaMV 35S promoter for LFY cDNA was constructed and transformed into Chrysanthemum morifolium (Ramat.) Tzvel. The result proved that the LFY cDNA contained 1263 base pairs in total length, encoding 420 amino acid. After Southern and Northern blot analyses the plants were grown in flowerpot, eight transgenic Ch. morifolium. plants were obtained. Compared with the normal plants, three of them flowered 65,67 and 70 days earlier and two of them delayed flowering 78 and 90 days, respectively.
基金
中国热带农业科学院青年基金
关键词
LFYcDNA
序列分析
转化
菊花
拟南芥
LFY cDNA, Cloning, Sequencing, Transformation, Chrysanthemum morifolium
