摘要
细胞凋亡（Ａｐｏｐｔｏｓｉｓ）或细胞程序性死亡（Ｐｒｏｇｒａｍｍｅｄｃｅｌｄｅａｔｈ，ＰＣＤ），最早被定义为一种有秩序、受控制并按某种预定程序发展的生理性自然死亡过程〔１〕。诱导细胞发生凋亡的因素很多，而病毒感染是常见的导致细胞凋亡的重要因素之一。由...
An extra copy
of p35 (p35 ORF and p35 EPO ) gene was introduced into the genome of TnNPV in order to
show their effects on very late gene expression. The p35 ORF and p35 EPO fragments
were oriented directly under the control of the synthetic and XIV promoters in the transfer vector
pSXIVVI +X3. Two strategies were followed to construct the p35 directly oriented recombinant
TnNPVs (TnNPV Δp10/LacZ + ΔHBVS/polh ++p35 ORF and TnNPV Δp10/LacZ +-ΔHBV
S/polh ++p35 EPO )either by inserting lacZ gene first into the viral genome or by inserting
directly oriented p35 first. The cells infected with TnNPV Δp10/lacZ + ΔHBV S/polh ++p35
ORF or TnNPV Δp10/lacZ +-ΔHBV S/polh ++p35 EPO showed no significant differences
in their survival ratios at the end of infection. The recombinant viruses produced less β
galactosidase activities than the control did, and the virus with early and late overexpression of
p35 produced the least β galactosidase. The result was comparable to that of the virus
production. Obviously, overexpression of p35 late or early and late affected TnNPV late and
very late gene expression. Both of them suggested that p35 does play a role in normal viral
replication.
出处
《病毒学报》
CAS
CSCD
北大核心
1999年第1期78-83,共6页
Chinese Journal of Virology
基金
国家自然科学基金
