摘要
目的:了解HBV感染的不同血清学组合的HBV-DNA阳性情况。方法:对324例血清同时进行ELISA方法测定HBV-M及荧光定量PCR法检测HBV-DNA。结果:HBsAg(+)组HBV-DNA阳性率为78.4%(254/324),HBsAg(-)组HBV-DNA阳性率为4.8%(5/103),两组间差异有统计学意义(P<0.05)。HBsAg(+)HBeAg(+)HBcAb(+)血清HBV-DNA阳性率为100.0%(120/120),平均含量为1.61×107拷贝/ml;HBsAg(+)HBeAb(+)HBcAb(+)血清HBV-DNA阳性率为47.0%(76/162),平均含量为3.42×104拷贝/ml;HBsAg(+)HBcAb(+)血清HBV-DNA阳性率为69.0%(29/42),平均含量为6.32×103拷贝/ml;HBsAb(+)HBeAb(+)HBcAb(+)血清HBV-DNA阳性率为5.2%(5/96),平均含量为4.12×101拷贝/ml。结论:定量PCR可真实反映HBV感染、复制及病程变化,对乙型肝炎临床诊断及治疗均有较大的指导意义。
Objective：To study the HBV-DNA positive rates under its different serological groups.Methods：324 HBV serum were detected by ELISA and quatitative PCR technique.Results：The HBV-DNA positive rate was 78.4%（254/324） in HBsAg positive group,and 4.8%（5/103） for HBsAg negative group,the differences between the two groups were statistically significant（P0.05）.100.0% positive rate of HBV-DNA was to HBsAg（＋） HBeAg（＋） HBcAb（＋） serum about average 1.61×107 copies/ml,47.0%（76/162） to HBsAg（＋）HBeAb（＋）HBcAb（＋） about 3.42×104 copies/ml,69.0%（29/42） to HBsAg（＋） HBcAb（＋） about average 6.32×103 copies/ml,5.2%（5/96） to HBsAb（＋）HBeAb（＋）HBcAb（＋） about average 4.12×101 copies/ml.Conclusion：Quantitative PCR can really reflect HBV infection and duplication and disease progress,it provides much more information for HBV diagnosis and therapy.
出处
《中国医药导报》
CAS
2010年第17期64-65,共2页
China Medical Herald
关键词
乙型肝炎病毒
DNA
ELISA
Hepatitis B virus
Deoxyribonucleic acid
Ensyme linked immunosorbent assay
