摘要
目的 评价结肠癌术后联合树突状细胞（DC）免疫治疗的临床疗效.方法 将40例接受结肠癌D3根治术并进行一次化疗的患者分为两组,一组在化疗后不进行DC免疫治疗;一组在术中采集新鲜肿瘤组织样本,并在化疗后进行自体肿瘤抗原负载DC免疫治疗.采集自体外周血,诱导制备自体肿瘤抗原负载的DC,进行4次免疫注射为1个疗程,前2次每周进行注射,后2次隔周进行注射.比较两组治疗前后细胞因子水平的变化,同时对治疗组进行迟发性超敏反应（DTH）检测及随访,观察患者的免疫应答与临床疗效.结果 制备获得成熟DC（mDC）,细胞活率〉95%,CD11c^＋CD14-细胞平均为90.24%±2.73%,CD11c^＋ HLA-DR^＋细胞平均为83.58%4±1.36%,CD80^＋细胞平均为87.92%±4.41%,CD83^＋细胞平均为62.48%±4.45%,CD86^＋细胞平均为97.72%±3.86%.治疗组患者细胞因子水平比对照组明显升高,差异有统计学意义（P〈0.05）,其中尤以IFN-γ升高最为明显（P〈0.01）.15例进行DTH检测的患者中有8例呈阳性,经过6～36个月随访,治疗组有1例患者复发,常规治疗组有1例复发,1例死亡.两组的中位无疾病进展期分别是22个月和17个月,差异有统计学意义（P〈0.05）.此外在DC治疗组中DTH阳性的患者无1例出现复发及转移.结论 结肠癌术后联合DC治疗可以改善患者机体的免疫功能,激发特异性免疫应答,取得了一定的临床疗效.
Objective To evaluate dendritic cell vaccine immunotherapy in postoperative colorectal cancer patients. Methods 40 colorectal cancer patients were divided into two groups. Group A was not treated with the dendritic cell vaccine after chemotherapy, Group B was treated with four courses of autologous dendritic cell vaccine. The level of cytokines was tested before and after the therapy in both groups. DTH were tested after the last course of treatment. Results The levels of cytokines significantly increased in group B after vaccination compared with that in group B before vaccination and that in group A.8 patients were DTH positive in 15 patients that were tested after the vaccination. The PTS （progression-free survival） was 22 months in group B compared with 17 months in group A. The recurrence of the disease was not observed in patients with DTH positive. Conclusion DC vaccine in postoperative colorectal cancer patients improves the immune status and elicits tumor-specific response.
出处
《中华普通外科杂志》
CSCD
北大核心
2010年第5期357-359,共3页
Chinese Journal of General Surgery
关键词
结肠肿瘤
树突细胞
免疫疗法
细胞因子类
Colorectal neoplasms
Dendritic cells
Immunotherapy
Cytokines
