摘要
目的研究半夏乙醇提取物的乙酸乙酯萃取部分,对其中的化学成分进行分离鉴定。方法采用硅胶、Sephadex LH-20柱色谱以及制备型高效液相色谱等手段进行分离纯化,通过理化性质和各种波谱技术对化合物进行结构鉴定。结果从半夏的乙酸乙酯萃取部分分离得到10个化合物,分别鉴定为环-(苯丙氨酸-酪氨酸)[cyclo-(Phe-Tyr),1]、环-(亮氨酸-酪氨酸)[cyclo-(Leu-Tyr),2]、尿嘧啶脱氧核苷(2′-deoxyuridine,3)、(+)异落叶松脂醇9-O-β-D-葡萄糖苷[(+)isolaricires-inol9-O-β-D-glucopyranoside,4]、(+)异落叶松脂醇[(+)isolariciresinol,5]、尿嘧啶(uracil,6)、没食子酸(gallic acid,7)、尿苷(uridine,8)、腺苷(adenosine,9)、环-(缬氨酸-酪氨酸)[cyclo-(Val-Tyr),10]。结论化合物1、3、4为首次从半夏属植物中分离得到,化合物2、7、10为首次从半夏中分离得到。
Objective To isolate and identify the chemical constituents of the ethyl acetate extract part of the bulk of Pinellia ternata（Thunb.）Breit.ethanol extract.Methods The compounds were isolated by silica gel column,Sephadex LH-20 column chromatography and HPLC techniques.And these compounds were identified by analysis of their spectral data and chemical properties.Results Ten compounds were obtained and identified as cyclo（Phe-Tyr）（1）,cyclo-（Leu-Tyr）（2）,2′-deoxyuridine（3）,isolariciresinol 9-O-β-D-glucopyranoside（4）,isolariciresinol（5）,uracil（6）,gallic acid（7）,uridine（8）,adenosine（9）and cyclo-（Val-Tyr）（10）.Conclusions Compounds 1,3,4 are all firstly isolated from the Pinellia genus.Compounds 2,7,10 are isolated from this plant for the first time.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2010年第6期429-433,共5页
Journal of Shenyang Pharmaceutical University
基金
国家自然科学基金资助项目(30873368)
关键词
半夏
环二肽
化学成分
结构鉴定
Pinellia ternata（Thunb.）Breit.
cyclodipeptide
chemical constituent
structure identification
