摘要
１９９７年４～５月，对花鲈（Ｌａｔｅｏｌａｂｒａｘｊａｐｏｎｉｃｕｓ）鱼苗期细菌性烂鳃和烂尾病的病原菌进行了研究。从具有明显症状的病鱼的病灶组织分离到２株致病菌Ｃ－１和Ｗ－１，经人工感染及从人工感染发病鱼体再分离的Ｗ－４和Ｗ－５菌株的再感染试验结果表明，所分离的细菌为鲈鱼苗烂尾，烂鳃病的致病菌。经６４项生物学特性，生理生化特征测试结果表明，这４株菌可归为一类，同属于鳗弧菌（Ｖｉｂｒｉｏａｎｇｕｉｌａｒｕｍ）。药敏试验结果表明，痢特灵、ＣＩＮ１００、ＭＥＬ２５、呋喃妥因、氯霉素、磺胺药＋ＴＭＰ和美洛西林等７种药物对病原菌有显著的抑制作用。
A serious epidemic of seaperch (Lateolabrax japonicus) fry occurred in some fish farms in Laizhou, Shandong Province. The typical symptoms of the diseased fry fish were poor swimming activities, darker body colour, anorexia or even rejection of food, congestive eyes, rotted gills and rotted caudal fins. A rod shaped bacterium was isolated from the diseased fish which has been proved to be the causative agent of the present infection of seaperch fry after experimental infection tests. Sixty four tests of morphological, physiological and biochemical characteristics of 4 isolated and reisolated strains were tested. All the 4 strains were rod shaped, motile with monotrichous flagella, gram negative, non luminescent. The bacteria grew well as faint yellow round colonies on TCBS agar. They were sensitive to vibristatic agent O/129 (150μg/mL), positive to Oxidase and Oxidation/Fermentation tests. They were classified to the same phenon by numerical taxonomy analysis and identified as V.anguillarum. Meanwhile, the sensitivity of two present strains to 47 chemotherapeutants were also investigated. Both strains had resistance to 22 kinds of chemotherapeutants and were more sensitive to Furazolidone, Cinoxacin, Mecillinam, Nitrofurantoin, Chloramphenicol, Sulphamethoxazole/Trimethoprim and Mezlocillin. Acknowledgements The authors thank FEBL, UNESCO and NSFC for their support of this research under contracts 9711B2(FEBL), 861.359.8 (UNESCO) and 39870581 (NSFC)
基金
淡水生态和生物技术国家重点实验室(FEBL)项目
联合国教科文组织(UNESCO)资助
国家自然科学基金
关键词
花鲈
鱼苗期
烂鳃
烂尾
鳗弧菌
鲈鱼
Seaperch(Lateolabrax japonicus)
fry stage, rotted gill
rotted caudal fins
Vibrio anguillarum
