摘要
目的:建立二至丸中齐墩果酸的含量测定的方法。方法:采用Venusil X B P-C18柱(4.6 mm×250 mm,5μm),以甲醇-0.4%乙酸铵溶液(83∶17)为流动相,检测波长210 nm,柱温25℃,流速1.0 mL.min-1。结果:齐墩果酸在0.100 4～1.807μg呈线性关系(r=0.999 8),平均回收率为99.7%(RSD 1.75%)。结论:此方法操作简便、可靠,重现性好,专属性强,能有效控制该制剂的质量。
Objective：To develop a RP-HPLC method to determine the contents of oleanolic acid in ErzhiPills.Method：The samples was separated on a Venusil X B P-C18 column（4.6 mm × 250 mm,5 μm）,using methanol-0.4 % ammonium acetate solution（83 ∶ 17） as the mobile phase with flow rate of 1 mL·min^-1 and detection at 210 nm.The column temperature was maintained at 25 ℃.Result：the linear range of oleanolic acid was at 0.1004 ～ 1.807 μg（r = 0.999 8）.The average recovery of oleanolic acid was 99.7%,RSD 1.75%（n=6）.Conclusion：The method is simple,accurate,and can be used for quality evaluation of Erzhi Pills.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第6期101-102,104,共3页
Chinese Journal of Experimental Traditional Medical Formulae
