摘要
[目的]用碳化二亚胺[1-ethyl-3-(3-dimethyl ami-nopropyl)carbodiimide,EDC]/N-羟基琥珀酰亚胺(N-hydroxysuccinimide,NHS)对Ⅱ型胶原海绵进行交联,评价了交联前后材料理化性能的改变,探讨其在软骨组织工程方面应用的可行性。[方法]提取Ⅱ型胶原后,通过真空冷冻干燥法冻干成多孔海绵材料后,室温下交联24h,再次冻干成为Ⅱ型胶原海绵支架材料。采用激光共聚焦显微镜、扫描电镜、拉力测试机等手段对海绵材料的结构及性能进行分析。将绿色荧光蛋白(GFP)标记的兔软骨细胞种植到交联后的Ⅱ型胶原支架材料后,观察细胞生长状况。[结果](1)交联后的海绵材料的力学性能与未交联组相比明显提高,达到(2.18±0.47)MPa;(2)交联组材料的抗胶原酶降解能力显著提高,4h后仅降解了8.28%。交联后孔径大小约为90μm,孔隙率和平衡含水率平均为93.39%和97.78%;(3)软骨细胞在Ⅱ型胶原海绵上生长状况良好。[结论]交联后的Ⅱ型胶原海绵保持其优良的理化特性和生物相容性,同时又有效地提高了力学性能和抗降解能力。
[Objective]Porous collagen type Ⅱ matrices crosslinked by 1-ethyl-3-（3-dimethyl aminopropyl）carbodⅡmide（EDC） and N-hydroxysucinimide（NHS） were developed as scaffolds for cartilage tissue engineering.[Method]Collagen type Ⅱ matrix was crosslinked by EDC/NHS,and then freeze-dried to achieve collagen scaffolds.The porous characteristics of the prepared scaffolds were tested by using diffenent methods,including laser scanning confocal microscopy （LSCM）,scanning electron microscopy （SEM） and mechanical testing machine.The chondrocytes labeled by GFP were planted in crosslinked collagen type Ⅱ,and observed by LSCM.[Result]（1） The mechanical properties of cross-linked matrices increased significantly,reaching 2.18±0.47MPa.（2）The degradation of cross-linked matrices decreased by 8.28% after 4 hours.The pore size was about 90μm,and their average porosity and water capacity reached 93.39% and 97.78%,respectively.3.Chondrocytes were in good condition in the crosslinked collagen.[Conclusion]The present work indicates that EDC/NHS-crosslinked collagen type Ⅱ could keep the properties and biocompatibility of collagen,besides,the mechanical strength increased and the degradation was decreased.It will be suitable for cartilage tissue engineering purposes.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2010年第12期1014-1018,共5页
Orthopedic Journal of China
基金
国家自然科学基金面上项目(编号:C160705)
广州市科技攻关项目(编号:200623-E0371)
广州医药卫生科技重点项目(编号:2006-Zdi-03
2006-ZDi-04)
关键词
Ⅱ型胶原
碳化二亚胺
N-羟基琥珀酰亚胺
交联
collagen type Ⅱ
1-ethyl-3-（3-dimethyl aminopropyl）carbodiimide
N-hydroxysuccinimide
crosslinking
