摘要
研究灰树花子实体多糖MT-α-glucan对自发性2型糖尿病模型KKay小鼠降血糖作用的量效关系和时效关系,对KKay小鼠空腹血糖、口服葡萄糖耐量、糖原合成的影响,以及对α-葡萄糖苷酶活性的影响。结果表明:随着MT-α-glucan给予剂量的增加,降血糖作用逐渐增强,以112、225、450mg/kgbw剂量组降血糖作用较为明显;MT-α-glucan 450mg/kgbw单次给予后8h降血糖作用最为明显,随后血糖水平逐渐恢复,给予后24h恢复到给予前水平。MT-α-glucan(450、150mg/kgbw)多次给予具有降低KKay小鼠空腹血糖、提高口服葡萄糖耐量、促进糖原合成的作用,并可使小鼠24h内随机血糖维持在较低的稳定水平。而且,MT-α-glucan对α-葡萄糖苷酶活性具有较强的抑制作用。因此,MT-α-glucan具有明显的降血糖活性,作用机理与抑制α-葡萄糖苷酶活性有关。
A kind of polysaccharide named MT-α-glucan was isolated and purified from the fruit body of Grifola frondosa.The dose-effect and time-effect relationships of MT-α-glucan were studied.The effect of MT-α-glucan on fasting blood glucose,24 h post-treatment blood glucose,oral blood glucose tolerance,muscle glycogen content in KKay diabetic mice and the activity of α-glucosidase in vitro were investigated.Results showed that MT-α-glucan at the dosages of 112,225 mg/kg bw and 450 mg/kg bw had significant hypoglycemic effect and the strongest hypoglycemic effect was observed at the dosage of 450 mg/kg bw at 8 h post-administration.However,blood glucose was restored to original level at 24 h post-administration.Consecutive administrations of MT-α-glucan at the dosage of 450 mg/kg bw and 150 mg/kg bw exhibited an obvious hypoglycemic effect on fasting blood glucose,blood glucose level at 24 h post-administration,and an increase effect on oral blood glucose tolerance and muscle glycogen content of KKay diabetic mice.In addition,MT-α-glucan had significant inhibitory effect onα-glucosidase activity in vitro.Therefore,MT-α-glucan had obvious hypoglycemic effect,which might be related to its inhibitory effect on α-glucosidase activity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第11期263-267,共5页
Food Science
关键词
灰树花
子实体
多糖
降血糖
Α-葡萄糖苷酶抑制剂
Grifolafrondosa
fruit body
polysaccharide
hypoglycemic effect
α-glucosidase inhibitor
