摘要
目的探讨集合淋巴结(PP结)及其淋巴细胞在实验性末端回肠炎发病中的变化及其意义。方法选用清洁级SD大鼠,随机分为造模组、缝线组、对照组,每组30只,分别予以回肠末端-盲肠侧侧吻合术、回肠末端缝手术线、仅麻醉不手术的处理。术后2、4、8周分批处死大鼠,取末端回肠组织,分别观察其病理学改变,及PP结及其淋巴细胞增殖和凋亡的情况并分析。结果 (1)造模组2、4周PP结个数和PP结淋巴细胞总数显著增加,8周时少量增加。(2)造模组2、4周PP结淋巴细胞少量凋亡,8周时大量凋亡,PP结淋巴细胞增殖情况与PP结个数变化相似。(3)造模组与缝线组8周均出现凋亡DNA的特征性"梯形"条带,且造模组200bp条带较缝线组明显。结论在实验性末端回肠炎造模2～8周内,PP结及其淋巴细胞呈现先增殖为主、后凋亡为主的变化。因此推测,持续的免疫反应在末端回肠炎发病过程中起着不可忽视的作用。
Objective To explore the changes of peyerg patches（PP） and its lymphocytes in experimental terminal ileitis and its significance. Methods Clean level SD rats were selected and divided into model group, suture group and control group randomly, 30 rats each. Then treat ileum-caecum side-to-side intestinal anatomists operation, suture in terminal ileum and nothing done respectively. 2,4 and 8 weeks later, kill these bats in batches. Finally get the terminal ileum to observe its pathological chan- ges, proliferation and apoptosis of PP and its lymphocytes, analyze the results in addition. Results （ 1 ） The number of PP and PP lymphocytes notably increased in model group at 2,4 weeks ,but little increased at 8 weeks. （2）The PP lymphocytes notably increased in model group at 2 and 4 weeks, but plenty at 8 weeks, whereas the proliferations of PP Iymphocytes were the same as changes of PP. （3）The apoptotic DNA characterized by ＂trapezoid＂ zones appeared in both model group and suture group at 8 weeks with more clear 200 bp zones appeared in the model group. Conclusion At 2 - 8 weeks of the experiment, PP and its lymphocytes firstly proliferate and finally apoptosis. Therefore, it is possible to infer that, the persistent immune reaaction may have effects can not be ignore in the course of terminal ileitis.
出处
《临床消化病杂志》
2010年第3期154-156,共3页
Chinese Journal of Clinical Gastroenterology
基金
湖南省衡阳市社会发展计划项目(2004-23)
