摘要
目的应用蛋白质组学技术分析模拟心理训练以及心理应激大鼠血清蛋白质谱的变化特点,鉴定差异表达蛋白,并据此评价心理训练效果。方法健康雄性Wistar大鼠32只,随机分为心理训练组、心理应激组、对照组,每组8只,其余8只作为应激源组。采用大鼠旁观电击模型,对应激源组大鼠进行间歇性足底电击刺激;心理应激组大鼠不受电击,通过视觉、听觉、嗅觉系统接收来自应激源组的信息,引起心理应激;心理训练组大鼠放入心理训练网状通道中进行训练,同时接受旁观电击的心理应激;对照组大鼠单独喂养,不接受任何处理。每天上午进行1次实验,持续2周。训练结束后采用表面加强激光解吸电离飞行时间质谱(SELDI-TOF-MS)芯片技术检测心理训练组、心理应激组、对照组大鼠血清蛋白表达谱的变化并进行统计学分析。结果方差分析显示三组血清中质荷比(M/Z)为7820.51、8870.18、6860.85、8625.13、11413.91Da的5种蛋白相对含量有显著差异(P<0.05)。与对照组比较,心理应激组M/Z为8870.18、6860.85、8625.13、11413.91Da的4种蛋白表达显著降低(P<0.05),心理训练组M/Z为7820.51Da的蛋白表达显著升高(P<0.05);与心理应激组比较,心理训练组M/Z为6860.85、7820.51、11413.91Da的3种蛋白表达显著升高(P<0.05)。结论心理训练能提高机体对心理应激的适应能力,使应激后低表达的蛋白明显上调。
Objective To investigate the changes of serum protein profile of rats in psychological stress and in analogous psychological training,identify the differential expression proteins,and assess the effectiveness of psychological training.Methods Thirty-two male Wistar rats were randomly divided into psychological training group,psychological stress group,control group and stressor group （8 each）.Rats in stressor group received intermittent foot-shock.Instead of foot-shock,the rats in psychological stress group animals received the stimulation information from stressor group by watching,listening and smelling.Rats in psychological training group were put in special equipment and received stimulation information from stressor group synchronously,and in control group were kept in individual cages and received no stress.Experiments were performed once a morning for two weeks.The changes of serum protein profile of rats in psychological training group,psychological stress group and control group were determined and statistically analyzed by Surface Enhanced Laser Desorption Ionization-Time of Flight-Mass Spectrometry （SELDI-TOF-MS） after the experiments.Results Variance analysis showed that the relative contents of proteins with M/Z value of 7820.51,8870.18,6860.85,8625.13 and 11413.91Da were significantly different （P〈0.05） among psychological training group,psychological stress group and control group.The expression of proteins with M/Z value of 8870.18,6860.85,8625.13 and 11413.91Da decreased in psychological stress group than in control group （P〈0.05）,while the expression of protein with M/Z value of 7820.51Da increased in psychological training group than in control group （P〈0.05）.The expression of proteins with M/Z value of 6860.85,7820.51 and 11413.91Da increased significantly in psychological training group than in psychological stress group （P〈0.05）.Conclusion Psychological training may improve the adaptability of rats to psychological stress,and up-regulate the expression of proteins down-regulated after stress.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2010年第7期886-888,共3页
Medical Journal of Chinese People's Liberation Army
基金
济南军区"十一五"科技研究课题(J115N025)
关键词
适应
心理学
应激
心理学
蛋白质组学
adaptation
psychological
stress
psychological
proteomics
