摘要
背景:空心微球材料具有很好的生物相容性,在生物医学和化学等许多领域得到广泛的应用。目的:分析Fe3O4空心磁性纳米微球制备流程中反应温度、反应时间、洗涤次数、超声时间工艺条件对粒径的影响。方法:以聚氧乙烯-聚氧丙烯-聚氧乙烯-嵌段共聚物F127为原料,用共沉淀法制备空心超顺磁性纳米微球。运用正交试验的数学方法设计一个4×3的正交实验模型,从反应温度、反应时间、洗涤次数、超声时间4个方面考察不同制备条件对空心磁性纳米微球粒径大小的影响。结果与结论:得到的最佳实验制备条件:在75℃下,充分反应6h,用甲醇/去离子水反复洗涤3次之后,用功率为30%的超声处理20min。样品通过0.22μm滤嘴过滤后,用粒径分布仪、X射线衍射和透射电子显微镜对产物进行表征观察,证实用最优的实验条件制备的空心Fe3O4磁性纳米微球粒径为37.5nm,外壳厚度10nm左右,颗粒大小均匀,分布较好。
BACKGROUND：Hollow microspheres exhibit good biocompatibility and have been widely used in many fields such as biomedicine and chemistry.OBJECTIVE：To study the affecting factors including the reaction temperature,reaction time,the number of washing,ultrasonic time in preparation process conditions on the particle size of hollow superparamagnetic Fe3O4 nanospheres.METHODS：With polyoxyethylene-polyoxypropylene-polyoxyethylene-block copolymer F127 as raw materials,Fe3O4 hollow superparamagnetic nanospheres were prepared by coprecipitation methods.Using mathematical methods of orthogonal experiment,a 4 × 3 orthogonal experimental model was designed.The influence of different preparation conditions such as the reaction temperature,reaction time,the number of washing and ultrasonic time,on the size of hollow magnetic nanoparticles was determined.RESULTS AND CONCLUSION：The optimal preparation conditions were：75 ℃,fully reacting 6 hours,washing three times using methanol/deionized water,30% power ultrasonic treatment for 20 minutes.After filtration by a 0.22-μm filter,the samples were characterized by particle size analyzer,X-ray diffraction and transmission electron microscopy.The average diameter of Fe3O4 hollow magnetic nanospheres was 37.5 nm,shell thickness was about 10 nm,particle size and distribution were even.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第25期4614-4618,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家"863"项目(2007AA021806)
项目名称:磁性空心球纳米药物制剂靶向治疗恶性肿瘤
国家"863"项目(2007AA021804)
项目名称:多功能金属壳热化疗纳米载药系统靶向治疗恶性肿瘤~~
