摘要
目的利用原核表达技术制备人肠道病毒71型(EV71)的亚单位融合疫苗,并通过小鼠模型对其保护效果进行评价。方法在大肠杆菌中融合表达EV71的亚单位肽段,随后将肽段免疫雌鼠,对其交配后产下的幼鼠进行病毒感染,通过检测其组织内的病毒拷贝数和病理变化,对疫苗的保护效果进行评价。结果通过肽段融合的方法,得到五种备选疫苗,分别为VacA,VacB,VacC,VacD和VacE,这五种疫苗均具有一定的体外保护能力,并且VacB和VacC与正常人大脑组织无明显交叉反应。动物模型评价结果显示,VacB和VacE能赋予幼鼠抗病毒感染的保护效果。结论利用EV71的小鼠感染模型评价了针对EV71的亚单位联合疫苗,VacB和VacE的保护效果较好,此外,VacB与人脑组织间无明显交叉反应,可以作为潜在的无神经毒性的临床使用疫苗。
Objective To prepare the fusion subunit vaccine candidates against human enterovirus 71 by over-expression in prokaryotes,and to evaluate the protection effect of that by mice infection model.Method Fusion subunit peptides of EV71 was over-expressed in Escherichia coli and applied in female mice immunity.The female mice were then sent to mate and the neonatal mice were infected by EV71.The validity of vaccine candidates was evaluated by monitoring the virus copy number and histopathology in mice tissue.Result Five vaccine candidates： VacA,VacB,VacC,VacD and VacE were prepared through peptides fusion expression in E.coli,and most of these candidates showed significant protection effect against EV71 infection in vitro.Meanwhile,VacB and VacC didn’t show any cross reactivity to human brain tissue.However,both VacB and VacE could confer the ability to defense EV71 infection.Conclusion The subunit vaccines for EV71 were evaluated by mice infection model,two candidates： VacB and VacE show perfect protection effect both in vitro and in vivo.Furthermore,VacB doesn’t show any cross reactivity to human brain tissue and can be used as potential no neurological toxicity vaccine for clinical therapy.
出处
《中国比较医学杂志》
CAS
2010年第6期7-12,86,87,共8页
Chinese Journal of Comparative Medicine
基金
实验动物技术平台(2009ZX10004-402)
