摘要
目的:观察参附注射液(Shenfu injection,SFI)对脑中动脉阻塞(middle cerebral artery occlusion,MCAO)所致小鼠缺血性脑损伤的作用及p38丝裂原激活蛋白激酶(p38MAPK)表达的影响。方法:60只健康雄性BALB/c小鼠(18g～22g,8周～10周)分为:A组为假手术加生理盐水处理组,B组为假手术加参附注射液处理组,C组模型组为造模加生理盐水处理组,D组参附组为造模加参附注射液处理组,每组15只。运用脑中动脉梗死缺血模型,结合神经行为学评价、氯化三苯基四氮唑(TTC)染色和蛋白印迹(Western blotting)技术,观察参附注射液对小鼠脑缺血损伤的影响以及皮质组织内p38MAPK磷酸化水平和蛋白表达量的变化。结果:研究发现,参附注射液可明显改善小鼠缺血后神经行为学表现,减小MCAO导致的鼠脑梗死面积;与假手术组相比,模型组小鼠皮质半影区p38MAPK磷酸化水平显著增高;参附组p38MAPK磷酸化水平明显降低。总p38MAPK在各组间差异不大。结论:参附注射液降低小鼠缺血性脑损伤,可能通过降低p38MAPK磷酸化水平而发挥神经保护作用。
Objective：To investigate the effects of Shenfu injection （SFI） on middle cerebral artery occlusion （MCAO） induced brain injury of mice and the phosphorylation of P38 mitogen activated protein kinase （ P38 MAPK） and protein expression levels in the ischemic cortex.Methods：Using MCAO mouse models, healthy male BALB/c mice were randomly divided into 4 group：group A（the sham operated control group）,group B（the sham operated trial group）,group C（the control group）and group D（the trial group）.The brain infarct volume and neural injury were determined by 2, 3, 5-Triphenyltetrazolium chloride （TTC） and neurological deficits evaluation.Western blot combined with Gel Doc imaging systems was applied to examine the changes of P38 MAPK phosphorylation and protein expression in murine brain.Results：SFI significantly decreased the neurological score, reduced infarction size.Ischemia increased the phosphorylation levels of p38MAPK significantly in penumbra of mouse brain （P0.05, n=6 for each group）.Furthermore, compared with the control, the phosphorylation levels of p38MAPK decreased more significantly in penumbra of trial ischemic group（P0.05, n=6）.However, there was no significant change in P38MAPK protein expression.Conclusion：p38MAPK signal pathway might be involved in neuroprotective effect of SFI on focal ischemic brain of mice.
出处
《现代生物医学进展》
CAS
2010年第11期2067-2069,共3页
Progress in Modern Biomedicine
基金
山东省医药卫生科技发展计划(2009HW006)
山东省教育厅2009年国内访问学者项目
