摘要
本文报告中药苦骨（sophorasubprostratakugua）根的提取物具有抗糖尿病并发症白内障的作用。这是苦骨提取物通过抑制葡萄糖代谢途径中的关键性酶—醛糖还原酶（AR）的活性而实现的。动力学研究显示苦骨提取物对底物DL-甘油醛和游离酶的结合为可逆性竞争性抑制；对辅酶NADPH为非竞争性抑制．苦骨提取物2．25mg／（kg·d）可以完全防止由链脲佐菌素（Streptozotocin，STZ）诱发的Ⅰ型糖尿病大白鼠的并发症白内障，其作用比依帕司他（Epalrestat）强20～40倍。苦骨提取物3mg／（kg·d）可使重症Ⅰ型糖尿病大白鼠的白内障症状得到改善。
In this paper, we reported the extract of Sphora susprostrata Kugua radix (also called Kugua extract)preyented marked1y the Streptozotocin-induced diabetic rats from getting cataracts. In practice, Kugua extract inhibited aldose reductase (AR ) that is a key enzyme of the pathway of glucose metabolism to convert glucose to sorbitol. In vitro,Human Recombinant AR was most effectively inhibited by Kugua extract,it has competitive inhibition on the Human Recombinant AR and on the (±) glyceraldehyde as substrate,and has non-competitive inhibition on the NADPH as coenzyme. It suggested that Kugua extract can be used to treat and prevent the diabetogenous cataract.
出处
《海峡药学》
1999年第1期15-17,共3页
Strait Pharmaceutical Journal
关键词
苦骨
提取物
白内障
糖尿病并发症
Kugua extract Anti cataract activity Kugua isoflavone glycosides Anti-diabetic and diabetogenous diseases
