摘要
目的：观察苦参碱对人肝细胞癌Hep3B细胞增殖、细胞周期、凋亡率以及细胞内β-catenin转录活性的影响,以探讨苦参碱抗肝癌细胞增殖作用的机制。方法：采用cell counting kit-8（CCK-8）细胞计数法测定细胞增殖活性;流式细胞术进行细胞凋亡率和细胞周期分析;双荧光素酶报告系统检测β-catenin的转录活性。结果：苦参碱（50~800mg/L）浓度依赖性地抑制Hep3B细胞的增殖（P〈0.01）,作用72h的半数抑制浓度（IC50）为312.53mg/L。阴性对照与400mg/L的苦参碱作用48h后,细胞凋亡率分别为4.39%和21.73%,G0/G1期细胞所占比例分别为57.39%和74.48%,S期细胞所占比例为27.67%和12.94%。50、200、800mg/L苦参碱浓度依赖性地抑制Hep3B细胞内β-catenin的转录活性（P〈0.05或P〈0.01）。结论：苦参碱通过抑制β-catenin的转录活性诱导Hep3B细胞凋亡和细胞周期阻滞,从而表现出抗肝癌细胞增殖的作用。
Objective：To investigate the effects of matrine on proliferation,cell cycle,apoptosis rate and β-catenin-dependent transcriptional activity in cultured hepatoma cell line Hep3B.Methods：Cell viability was analyzed by cell counting kit-8（CCK-8）assay.Cell cycle and apoptosis rate wene determined by flow cytometry analysis,β-catenin-dependent transcriptional activity was assayed with Dual-Luciferase Reporter System.Results：72 h of matrine（50~800 mg/L）exposure significantly suppresses Hep3B cells growth in a concentration-dependent manner（P0.01）,the 50 percent inhibitory concentration（IC50）was 312.53 mg/L.A higher proportion of apoptotic cells in matrine-treated group than in control groups（21.73±1.66% vs.4.39±1.93%）are shown.Cell proportions in G0/G1 phase were respectively 74.48% and 57.39% in matrine-treated group and control group.Cell proportions in S phase wene respectively 12.94% and 27.67%.β-catenin reporter activity was also decreased by matrine treatment in a concentration-dependent manner in Hep3B cells（P0.05 or P0.01）.Conclusion：Through inhibition of β-catenin-dependent transcription,matrine induces cell cycle arrest and apoptosis,and subsequently suppresses proliferation of hepatoma cells.
出处
《中药材》
CAS
CSCD
北大核心
2010年第5期778-781,共4页
Journal of Chinese Medicinal Materials
基金
广东省自然科学基金(07300451)
广东省自然科学基金(8151051501000020)
广东省医学科研基金(A2008376)
南方医院院长基金面上项目(20080034)
