摘要
为了建立烟草病毒病的分子鉴定体系,采用TMV,CMV和PVY3种病毒的检测引物对烟草花叶病样品进行了RT-PCR扩增,并从核酸水平上对病毒基因序列进行了分析。RT-PCR鉴定结果显示,该病害病原为TMV,测序结果显示扩增产物长923bp,序列比对后发现该序列与TMV3个中国分离物的同源性高达97%,序列分析结果表明该段序列位于TMV基因组3'末端,包含部分MP基因、完整CP基因和3'端非编码区。对CP基因进行了系统进化分析,且该序列已成功登陆GenBank。
In order to establish a system for molecular identification of tobacco virus diseases,primers for TMV,CMV and PVY detection were used in RT-PCR for TMV samples,and the genetic sequence of the amplified product was analyzed at nucleic acid molecular level. The results of RT-PCR indicated that the pathogen was TMV and the amplification product was 923 bp in length. NCBI BLAST showed that it shared 97% sequence identity with 3 China isolates. Sequence analysis indicated that the fragment located at the 3＇ terminal of TMV genome and included a part of movement protein （ MP） gene,complete coat protein （ CP） gene and 3＇ terminal’s non-coding region. The phylogenetic tree analysis of CP gene was conducted,and the sequence has been logged in GenBank.
出处
《烟草科技》
EI
CAS
北大核心
2010年第7期58-61,共4页
Tobacco Science & Technology
基金
院长科技发展基金项目"植物源抗病毒抑制剂的筛选研究"(122008CA0390)
郑州烟草研究院科技项目"烟草主要病毒病分子变异监控及追踪技术研究"(122009CZ0410)
关键词
烟草
病毒
分子鉴定
进化树分析
Tobacco
Virus
Molecular identification
Phylogenetic tree analysis
