摘要
目的:研究转筋草Pachysandra terminalis中的酚类化合物及其抗氧化活性。方法:综合运用硅胶柱色谱、凝胶柱色谱、HPLC制备色谱等多种色谱法分离纯化转筋草中的化学成分;采用NMR等波谱学方法鉴定化合物结构;进一步通过DP-PH法评价各化合物的抗氧化活性。结果:从转筋草乙酸乙酯层中分离得到9个酚类化合物,分别为对羟基苯甲醛(1),香草醛(2),3-甲氧基-4-羟基苯乙酮(3),丁香醛(4),水杨酸(5),对羟基苯甲酸(6),阿魏酸(7),2,3,4-三羟基苯甲酸(8),原儿茶酸(9)。抗氧化实验结果表明:化合物1～9均有较强的抗氧化活性,其中化合物7～9在50μmol.L-1时对DPPH自由基的清除能力分别为87.8%,97.8%和92.0%。结论:所有化合物均为首次从该属植物中分离得到;均具有较强的抗氧化活性。
Objective： To study the phenol constituents from Pachysandra terminalis and their antioxidant activities.Method： Constituent isolation and purification was carried by repeated column chromatography（silica gel,Toyopearl HW-40 and preparative HPLC）,and their structures were elucidated on the basis of spectral data analysis.DPPH method was used to evaluate the free radical scavenging activity of the isolated compounds.Result： Nine phenol compounds（1-9） were isolated and their structures were identified as follow： p-hydroxybenzaldehyde（1）,vanillin（2）,1-（4-hydroxy-3-methoxyphenyl）-ethanone（3）, syringaldehyde（4）,salicylic acid（5）,p-hydroxybenzoic acid（6）,ferulic acid（7）,2,3,4-trihydroxybenzoic acid（8）,3,4-dihydroxybenzoic acid（9）.The isolated compounds showed obviously antioxidant activity.At the concentration of 50 μmol·L-1,compounds 7-9 revealed DPPH free radical scavenging rates were 87.8%,97.8% and 92%,respectively.Conclusion： Compounds 1-9 were isolated from this genus for the first time.They showed the significant antioxidant activity.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第14期1820-1823,共4页
China Journal of Chinese Materia Medica
基金
天津市基础医学研究中心PI科研基金
关键词
板凳果属
转筋草
酚类化合物
抗氧化活性
Pachysandra
Pachysandra terminalis
phenol
antioxidant activity
