摘要
目的建立宁夏黄芪HPLC-UV色谱指纹图谱,为黄芪药材的鉴别提供依据,并为宁夏黄芪药材的标准化种植提供实验数据。方法采用HPLC-UV方法对宁夏16种不同来源的黄芪药材建立指纹图谱。色谱柱Thermo ODS-2HYPERSIL(250mm×4.6mm,5μm);流动相甲醇-乙腈-醋酸-醋酸钠(pH=4.5)缓冲液;检测波长260nm;流速1ml/min;柱温25℃。结果建立了宁夏黄芪药材HPLC-UV指纹图谱,标定出23个共有峰,计算出各批次药材之间的相似度。结论各批黄芪药材指纹图谱相似度大小与其来源相关,建立的指纹图谱可作为黄芪药材的鉴定方法和宁夏黄芪药材标准化种植的实验依据。
Objective To establish chromatographic fingerprint of Radix Astragali by HPLC which can be used to Radix Astragali＇s identity and standard planting.Methods A method of HPLC-UV was applied to establish chromatographic fingerprint of 16 Radix Astragali samples from different region and growth in Ningxia.The chromatographic condition：Thermo ODS-2HYPERSIL（250mm × 4.6mm,5μm） column,the mobile phase consisted of methanol,acetonitrile,and sodium acetate-acetic acid buffer（PH = 4.5） which was in the gradient elution at a flow rate of 1.0 mL.min-1,at 25℃.Results Established the chromatographic fingerprint of Radix Astragali in Ningxia,23 peaks were identified as the characteristic fingerprints of Radix Astragali.The similarity of Radix Astragali from different region and growth was calculated.Conclusion The similarity of Radix Astragali is related with its＇region and growth.This fingerprint is useful for the identity and standard planting of Radix Astragali.
出处
《宁夏医学杂志》
CAS
2010年第7期597-599,I0001,共4页
Ningxia Medical Journal
基金
宁夏自治区"十一五"科技攻关项目(2006074)
