摘要
[目的]筛选铁皮石斛离体培养开花诱导的适宜激素因子。[方法]采用铁皮石斛茎段离体培养的试管苗为试验材料,以MS为基本培养基,进行单因子激素处理(PP333、TDZ的不同浓度梯度)和多因子激素处理(PP333、TDZ、6-BA以及NAA的不同组合)的比较试验,研究激素因子对铁皮石斛离体培养开花诱导的效应。[结果]单因子激素处理中,PP333、TDZ的适宜浓度和其诱导的花芽形成率分别为0.2 mg/L和8.5%、0.06 mg/L和15.5%;多因子激素处理对开花诱导的效应依次为:PP333+6-BA+NAA+TDZ组合处理>PP333+6-BA+NAA组合处理>PP333+6-BA组合处理和PP333+NAA组合处理;适宜的激素组合为PP3330.3 mg/L+6-BA 0.5 mg/L+NAA 0.5mg/L+TDZ 0.06 mg/L,其花芽形成率和花开放率高达80.4%和90.3%。[结论]PP333和TDZ对铁皮石斛离体培养开花诱导具有重要影响,TDZ的效应大于PP333。适宜的TDZ浓度与其他激素的合理配比,有利于花芽的形成。
[Objective] The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method] The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium,comparative tests were carried out between single-factor hormone treatments（different concentrations of PP333 and TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Result] Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,that of TDZ treatment were 0.06 mg/L and 15.5%;The effects of multi-factor hormone treatments on the flowering induction were ordered as follows：（PP333＋6-BA＋NAA＋TDZ）〉（PP333＋6-BA＋NAA）〉（PP333＋6-BA） and（PP333＋NAA）;The most suitable treatment was PP333 0.3 mg/L ＋6-BA 0.5 mg/L＋ NAA 0.5 mg/L＋TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion] PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo,and the effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.
出处
《安徽农业科学》
CAS
北大核心
2010年第16期8308-8311,共4页
Journal of Anhui Agricultural Sciences
基金
广西科技厅计划项目(桂科攻0322024-3A)
广西留学回国人员科技项目
关键词
铁皮石斛
离体培养
激素因子
开花诱导
Dendrobium officinate Kimura et Migo
culture in vitro
Hormone factor
Flowering induction
