摘要
通过在鲫日粮中添加稀土壳聚糖螯合盐(rareearth-chitosan chelate,RECC),研究其对鲫(Carassius auratus L.)肠道发育的影响。试验分为1个空白对照组和3个试验组,日粮中稀土壳聚糖螯合盐的添加量分别为0.00%、0.08%、0.16%和0.24%。每组设3个重复,每个重复放养30尾鲫。试验鲫初始平均体重为(5.04±0.08)g。每日投喂2次(9:00,16:00),每次投喂量为每池鱼总重的0.5%～1.0%(投喂量据具体情况调节),养殖期60d。试验期间水温(26.7±2.5)℃,自然光照,水体pH为7.1～7.8,溶氧>4mg/L。研究结果表明:添加RECC能改善鲫肠道形态结构。添加RECC后肠道粘膜褶变细长,形状较均一,数目增多。与空白对照组相比,0.08%RECC组粘膜褶数目显著增加(P<0.05)、粘膜褶宽度显著降低(P<0.05)、杯状细胞数目显著增多(P<0.05);0.16%RECC组杯状细胞数目极显著增多(P<0.01)。超显微观察发现,空白对照组肠道微绒毛分块,分布较稀疏且不均匀;而RECC添加组微绒毛较空白对照组明显密集,且分布均匀。
A feeding study was performed to investigate possible performance enhancing effects of the rare earth-chitosan chelate （RECC） on intestinal structure of Carassius auratus L. Three hundred and sixty individuals （about 5.0 g） were allotted to four dietary treatments： a control group and three RECC-treated groups. RECC-treated groups were supplemented with 800 mg,1 600 mg and 2 400 mg of RECC per kg feed respectively. Each group had three duplicates. The whole feeding period lasted for sixty days. Results are as follows： RECC added in diet promoted the development of intestine. It was found that RECC-treated groups had higher numbers of plicamucosa than the control group,and plicamucosas became longer and narrower. Compared with the control group,the 0.08% RECC group had a greater number of plicamucosa （P0.05）,a lower width of policamucosa （P0.05）,and a higher number of goblet cells （P0.05）; the 0.16% RECC group had a greater number of goblet cell （P0.01）. Scanning electron microscope observation showed that microvilli in RECC-treated groups were more uniform and denser than the control group.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2010年第4期475-481,共7页
Journal of Shanghai Ocean University
基金
湖北省教育厅重点项目(D200618007)
关键词
稀土壳聚糖螯合盐
肠道发育
鲫
rare earth-chitosan chelate
intestinal structure
Carassius auratus L.
