摘要
目的：建立高效液相色谱法测定多维元素泡腾片中5种B族维生素的含量。方法：以Inertsil ODS-3 C1 8（250 mm×4.6mm,5μm）为分析柱,乙腈-0.003 mol·L^-1己烷磺酸钠溶液为流动相进行梯度洗脱,流速为1 mL·min^-1;检测波长为277nm;柱温为40℃。结果：维生素B1、维生素B2、维生素B6、烟酰胺、叶酸的浓度分别在8.45-50.7μg·mL^-1（r=0.9997）、9.27-55.6μg·mL^-1（r=0.9997）、11.1-66.5μg·mL^-1（r=0.9998）、91.4-548μg·mL^-1（r=0.9998）、0.85-5.09μg·mL^-1（r=0.9997）范围内与峰面积呈良好的线性关系。平均回收率（n=9）分别为100.7%,98.5%,99.8%,100.1%,100.4%。重复性的RSD（n=6）分别为0.9%,0.9%,1.3%,1.1%,1.2%。结论：该法专属性好,操作简便,准确度高,可作为该产品质量控制的方法。
Objective：To establish an HPLC method for the determination of vitamin B complex in multivitamin effervescent tablets.Methods： The separation was performed on an Inertsil ODS-3 C18 column（250 mm×4.6 mm,5 μm）;a gradient elution procedure was composed of acetonitrile-0.003 mol·L^-1 1-hexanesulfonic acid sodium salt solution,and the flow rate was 1.0 mL·min-1;detective wavelength was set at 277 nm.The separation was performed at 40 ℃.Results：Vitamin B1,vitamin B2,vitamin B6,nicotinamide,folic acid had good linearity in the range of 8.45-50.7 μg·mL^-1（r=0.9997）,9.27-55.6 μg·mL^-1（r=0.9997）,11.1-66.5 μg·mL^-1（r=0.9998）,91.4-548 μg·mL^-1（r=0.9998）,0.85-5.09 μg·mL^-1（r=0.9997）,respectively.The average recoveries（n=9） were 100.7%,98.5%,99.8%,100.1%,100.4%,respectively.RSDs of repeatability（n=6） were 0.9%,0.9%,1.3%,1.1%,1.2%,respectively.Conclusion：The method is reproducible,simple and accurate.It can be used for the determination of vitamin B complex in multivitamin effervescent tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第7期1271-1275,共5页
Chinese Journal of Pharmaceutical Analysis
