摘要
目的：建立高效液相色谱-蒸发光散射检测（HPLC-ELSD）法测定聚乙二醇4000散剂中聚乙二醇4000的含量。方法：采用Venusil XBP-C8色谱柱（150 mm×4.6 mm,5μm）,以甲醇-水为流动相进行梯度洗脱,流速1.0 mL·min^-1,柱温35℃;Alltech ELSD-2000检测器,漂移管温度：80℃,载气为空气,流速：2.8 L·min^-1。结果：聚乙二醇4000线性范围为0.5-1.5 mg·mL^-1（r=0.9995）,回收率为101.3%。结论：本方法准确、简便、快速,重复性好,可用于聚乙二醇4000散剂的含量测定。
Objective：To approach an assay method of determination for macrogol 4000 in macrogol 4000 powder by HPLC-ELSD method.Methods：The assay was performed on Venusil XBP-C8 column（150 mm×4.6 mm,5 μm）with a gradient program of methanol-water as the mobile phase and an Evaporative Light-Scattering Detector（Alltech ELSD-2000） as the detector.The drift tube temperature was 80 ℃,the gas flow rate was 2.8 L· min^-1.Column temperature： 35 ℃.Results：The line ranges of macrogol 4000 was 0.5-1.5 mg·mL^-1（r=0.9995）.The average recoveries（n=9） of macrogol 4000 was 101.3%.Conclusion：The method is proved to be rapid,accurate and convenient,and provided a reliable way for evaluating the quality of macrogol 4000 powder.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第7期1276-1278,共3页
Chinese Journal of Pharmaceutical Analysis
