摘要
用流式细胞仪技术和ＭＴＴ法观察了云芝糖肽（ＰＳＰ）对由植物血凝素（ＰＨＡ）诱导的人外周血淋巴细胞（ＰＢＬ）增殖和Ｔ细胞亚群变化的调节作用。结果表明，５０～１００μｇ／ｍＬ ＰＳＰ对经ＰＨＡ诱导的人 ＰＢＬ增殖有显著的促进作用（Ｐ＜０． ０５），该浓度的ＰＳＰ也能促进细胞从Ｇ_１期进入Ｓ期，并可使ＣＤ_4^+细胞明显升高，使ＣＤ_4^+／ＣＬ_8细胞比例增加，提示ＰＳＰ具有免疫促进作用。
Regulatory effect of polysaccheriopeptide (PSP) on human peripheral blood lymphocyte (PBL) proliferation and T lymphocyte subpopulation was studied. The results showed that: phytohemagglutinin (PHA)-activated human PBL could increase proliferation especially after treated with PSP in concentrations of 50-100 μg/mL. In this study, the effect of PSP on cell cycling induced by PHA was investigated, DNA synthesis measured by a flowcytometer was shown to be in- creased but not in a dose-dependant manner. Cell cycling analysis demonstrated that the increment was main- ly at the stage when cells enter S phase. It was observed that in PSP treated cases CD_4^+ cell group increased, suggested that PSP has certain im- munoregulatory effect in improving proliferation of activated human PBL, and increasing the CD_4^+cell group.
出处
《中草药》
CAS
CSCD
北大核心
1999年第1期37-39,共3页
Chinese Traditional and Herbal Drugs
关键词
云芝糖肽
人外周血淋巴细胞
增殖
T细胞亚群
polysacchariopeptide human peripheral blood lymphocyte T lymphocyte subpopulation
