摘要
目的：探讨胶束纸色谱法在分离、鉴别黄酮类及其水溶性衍生物中的应用。方法：采用高于临界胶束浓度（ＣＭＣ）的十二烷基硫酸钠ＳＤＳ溶液作展开剂，通过纸色谱分离和检测槲皮素、金雀异黄素及其硫酸酯衍生物，考察了ＳＤＳ胶束浓度、有机改性剂及浓度、荧光强度对分离效果的影响。结果：０．０１～０．０２ｍｏｌ·Ｌ－１的ＳＤＳ胶束水溶液为适宜的胶束浓度，加入体积分数为２％～４％的异丙醇或正戊醇可以显著地改善样品分离效果；ＳＤＳ溶液也使样品的荧光强度得到肯定的增强。结论：ＳＤＳ胶束纸色谱法可以简便、快速、准确。
OBJECTIVE:To investigate the use of micellar paper chromatography on the isolation and identification of flavonoids and their soluble derivatives.METHOD:Quercetin,Genistein and their sulphate derivatives were separated and identified by SDS solution above critical micelle concentration (CMC) on paper chromatography.The concentration of SDS,ratio of organic modifier and their concentration,fluorescence were investigated.RESULTS:The best concentration of SDS micelle was 0.01～0.02mol·L -1 ,and the spot effects of micellar chromatography could be improved by adding 2%～4% isopropanol (or n amyl alcohol)into the mobile phase.CONCLUSION:The simple,rapid,accurate and qualitative method could be used to analyze and isolate flavonoids and their soluble derivatives.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
1999年第3期180-182,共3页
Chinese Pharmaceutical Journal
基金
广东省重点学科专项基金
广东医学院青年科学基金
关键词
胶束纸色谱
槲皮素
金雀异黄素
硫酸酯衍生物
micellar paper chromatography,quercetin,genistein,flavonoid sulphate,SDS,modifier
