摘要
目的:研究甘草中黄酮类化合物异甘草素、甘草素、异甘草苷、甘草苷的体外抗肿瘤作用。方法:SRB法测定4种化合物对白血病HL-60、人纤维肉瘤HT-1080、肝癌BEL-7402细胞增殖的影响及半数抑制浓度IC50;吖啶橙/溴化乙锭(AO/EB)双荧光染色法观察肿瘤细胞经加药处理后的形态变化。结果:异甘草素对3种肿瘤细胞的增殖均有较强的抑制作用,甘草苷仅对HL-60细胞有较显著的抑制作用,而异甘草苷和甘草素对3种肿瘤细胞增殖的抑制作用较弱。结论:甘草黄酮化合物抗肿瘤活性可能与其母环开、裂及其侧链羟基数目相关,异甘草素具有较好的体外抑瘤作用,是一种有潜力的抗癌药物,值得进一步深入研究。
Objective：The aim of this study was to investigate the antitumor effects of Isoliquiritigenin,Liquiritigenin,Isoliquiritin and Liquiritin in vitro.Methods：The proliferation and IC50 of four compounds on HL60,HT-1080,BEL-7402 cancer cell lines were assayed by SRB method in vitro.The morphology changes of tumor cells treated by drugs were observed by AO/EB fluorescence staining.Results：The inhibition effects of isoliquiritigenin on all three tumor cell lines were significant.Liquiritin inhibited proliferation of HL-60 cell obviously,but liquiritigenin and isoliquiritin has less toxicity to all three tumor cell lines.Conclusion：The antitumor activity of four licoflavones may be related to its mother rings crazing and hydroxy amounts on its side chains.Our results showed that isoliquiritigenin possess strong effects of inhibiting tumor cell proliferation in vitro,it may be a potential anti-cancer drug and need study further.
出处
《食品科技》
CAS
北大核心
2010年第7期88-92,共5页
Food Science and Technology
基金
国家自然科学基金项目(30960451)
关键词
异甘草素
甘草素
异甘草苷
甘草苷
抗肿瘤
isoliquiritigenin
liquiritigenin
isoliquiritin
liquiritin
antitumor
