摘要
目的建立测定红霉素、琥乙红霉素及依托红霉素活性的浊度法,并对其进行考察评价。方法以金黄色葡萄球菌为试验菌,加菌量为1.0%～1.2%(V/V),(37±0.5)℃培养3～4 h后测定。结果红霉素抗生素线性浓度为0.08～0.8 IU/mL。结论该方法灵敏、快速、影响因素较少,可作为测定、评价红霉素、琥乙红霉素及依托红霉素活性的方法。
Objective To establish a turbidimetric method for determining the antimicrobial activity of erythromycin and erythromycin ethylsuc-cinate and erythromycin estolate.Methods The test organism was Staphylococcus aureus and cultured with fresh medium（pH = 7.0）.Staphylococcus aureus inoculated with a 1.0%-1.2%（V/V） ratio was cultured at（37 ± 0.5）℃ for about 3-4 h.Results The linear range of erythromycin was 0.08-0.8 IU/mL.Conclusion The method is sensitive,rapid with less influencing factors,which is feasible to appraise the antimicrobial activity of erythromycin,erythromycin ethylsuccinate and erythromycin estolate.
出处
《中国药业》
CAS
2010年第14期40-42,共3页
China Pharmaceuticals
基金
2006年河北省科技厅资助课题
项目编号:06276445
关键词
抗菌活性
浊度法
红霉素
琥乙红霉素
依托红霉素
antimicrobial activity
turbidimetric method
erythromycin
erythromycin ethylsuccinate
erythromycin estolate
