摘要
背景:体质量指数(BMI)是研究肥胖的常用表型,它是由多基因决定的复杂性状。目的:验证骨钙素基因HindⅢ多态性是否与体质量指数的变异相关联。方法:征集南昌当地绝经前女性390例,并测量身高和体质量。用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法对所有个体的骨钙素基因HindⅢ位点进行基因分型。结果与结论:骨钙素HH,Hh和hh基因型频率分别为0.077,0.408和0.515。骨钙素基因型分布符合哈的温格尔平衡(P>0.05)。骨钙素基因HindⅢ与体质量指数存在显著的关联(P=0.002),它可以解释大约5.47%体质量指数的变异。HH基因型个体的体质量指数最高[(22.81±0.73)kg/m2],Hh基因型个体的体质量指数居中[(21.50±0.53)kg/m2],而hh基因型个体的体质量指数最低[(20.23±0.63)kg/m2]。因此,HH和Hh基因型个体的体质量指数比hh基因型个体的体质量指数分别高大约12.75%和6.28%。文章首次在健康绝经前女性中报道骨钙素基因HindⅢ多态性与体质量指数的变异相关联。
BACKGROUND： Body mass index （BMI） is a commonly used phenotype for obesity, which is determined by multiple genetic factors. OBJECTIVE： To investigate whether osteocalcin （also known as bone Gla protein, BGP） Hind Ⅲ genetic polymorphism is associated with BMI variation. METHODS： A total of 390 premenopausal women from a local population of Nanchang City were selected. Body weight and height were measured. All participants were genotyped at the BGP Hind Ⅲ locus using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. RESULTS AND CONCLUSION： The BGP genotype frequencies of HH, Hh and hh were 0.077, 0.408 and 0.515, respectively. The distribution of BGP Hind Ⅲ genotypes was consistent with Hardy-Weinberg equilibrium （P〈0.05）. The BGP Hind Ⅲ were significantly associated with BMI （P=0.002）, which could explain about 5.47% of BMI variation. On average, BMI of individuals with HH genotype was the highest [（22.81±0.73） kg/m2], individuals with Hh genotype was intermediate [（21.50±0.53） kg/m2], while individuals with hh genotype was the lowest [（20.23±0.63） kg/m2]. Therefore, carriers of HH and Hh genotypes had, respectively, approximately 12.75% and 6.28% higher BMI than carriers of the hh genotype. To our best knowledge, this is the first study reporting the association of BGP Hind Ⅲ genetic polymorphism and BMI in healthy premenopausal women.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第28期5317-5320,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
the National Natural Science Foundation of China, No. 30760071, 30960118
Foundation of the Education Department of Jiangxi Province, No. GJJ08106
Science and Technology Research Program of Nanchang University, No. J06~~
