摘要
报道了生姜茎尖离体培养的研究结果．结果表明：初代及继代培养的基本培养基以Ｎ６为宜；ＫＴ对芽的增殖效果较佳；ＢＡ对芽没有明显的增殖效果，而且对芽的生长有毒害作用；ＫＴ５．０～７．５ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ的激素配比对丛生芽的诱导效果较好，２５～３０ｄ可增殖３．３～３．９倍；０．５ｍｇ／Ｌ的ＮＡＡ和ＩＢＡ均能有效刺激试管苗生根，生根率高达１００％；表土是良好的移栽基质，成活率为９３．３％．
This paper deals with the shoot tip culture of common ginger (Zingiber officinale Rosc.) in vitro .The experimental results show as follows:The basal medium N 6 is suitable for the initial culture and subculture;KT exerts an ideal effection on the shoot proliferation;BA has no obvious proliferation effect on the shoots, and it is harmful to the growth of shoots; the hormone scheme of 5.0～7.5 mg·L -1 KT + 0.5 mg·L -1 NAA exerts a good effect on the clustered shoots formation, and the shoot are proliforated for 3.3～3.9 times in 25～30 days;0.5 mg·L -1 NAA(or IBA) can effectively promte the root emergence of the shoots, and the percentage of the root emergence amounts to 100%;the surface soil is a good kind of basal material for the transplantation, and the survial rate amounts to 93.3%.
出处
《海南大学学报（自然科学版）》
CAS
1999年第1期59-63,共5页
Natural Science Journal of Hainan University
关键词
姜
茎尖
离体培养
组织培养
Common ginger (Zingiber officinale)
shoot tip
culture in vitro
