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TUNEL法检测类猪圆环病毒2型因子P1诱导猪免疫器官细胞凋亡的研究 被引量：19

Apoptosis in Immune Organs of Pigs Induced by Porcine Circovirus Type 2-like Agent P1
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摘要 研究类猪圆环病毒2型因子P1体内诱导的细胞凋亡作用。将P1分子克隆接种普通仔猪,用原位末端标记技术(TUNEL)检测猪的扁桃体、脾脏、腹股沟淋巴结等免疫器官细胞的凋亡情况。猪感染P1后21和35 d的扁桃体、脾脏和腹股沟淋巴结等免疫器官细胞出现了凋亡。P1可能引起猪的免疫抑制。 The research is to study the apoptosis of pigs induced by porcine circovirus type 2-like agent P1.The apoptosis was detected by TUNEL assay in immune organ samples（tonsil,spleen,superficial inguinal lymph node） of pigs infected by P1 molecular clone.The results showed that the apoptosis-specific signal was detected in immune organ samples（tonsil,spleen,superficial inguinal lymph node） at 21 and 35 d after P1 infection.The result suggests that P1 appears to be related to immunosuppression resulted in apoptosis of lymphocyte.
作者 温立斌 何孔旺 杨汉春 王玉然 董美响
机构地区 江苏省农业科学院兽医研究所 中国农业大学动物医学院 河北省动物卫生监督所 河北省饲料工业协会
出处 《华北农学报》 CSCD 北大核心 2008年第B10期88-91,共4页 Acta Agriculturae Boreali-Sinica
基金 国家“973”计划前期研究专项资助课题(2007CB116308) 江苏省博士后基金(5910602)
关键词 猪圆环病毒2型 P1 凋亡 TUNEL Porcine circovirus type 2 P1 Apoptosis TUNEL
分类号 S852.65 [农业科学—基础兽医学]
  • 相关文献

参考文献19

  • 1Allan G,Meehan B,Todd D,et al.Novel porcine circovirus from pigs with wasting disease syndrome[J].Vet Rec,1998,142:467-468.
  • 2Ellis J,Hassard L,Clark E,et al.Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome[J].Can Vet J,1998,39:44-51.
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二级参考文献25

  • 1ELLIS J,HASSARD L,CLARK E,et al.Isolation of circoviurs from lesions of pigs with postweaning multisystemic wasting syndrome[J].Can Vet J,1998,39(1):44-51.
  • 2ROSELL C,SEGALES J,RAMOS-VARA J A,et al.Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome[J].Vet Rec,2000,146(2):40-43.
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  • 10ALLAN G, MEEHAN B, TODD D, et al. Novel porcine circovirus from pigs with wasting disease syndrome[J]. Vet Rec, 1998, 142(17): 467-468.

共引文献131

同被引文献114

引证文献19

二级引证文献50

华北农学报
2008年 第B10期

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