摘要
为了进一步探讨运动心脏重塑的发生机制，以大鼠１８Ｓ－ＲＮＡ为内参照，采用先进的定量反转录聚合酶链反应（ＱＲＴ－ＰＣＲ）技术，对２０只经１２周耐力训练的 ＳＤ大鼠心脏中原癌基因ｃ－ｍｙｃ的表达进行了研究。结果表明，与安静对照组相比，耐力训练组大鼠心室中ｃ－ｍｙｃ的表达非但没有提高，反而出现非常显著的下降（Ｐ＜０．００１）；尽管训练组大鼠心房中ｃ－ｍｙｃ的表达较对照组有明显升高，但无显著性统计学意义（ｐ＜ ０．０５）。训练组大鼠。心室中 ｃ－ｍｙｃ的表达显著低于心房中 ｃ－ｍｙｃ的表达（Ｐ＜ ０．０１），而安静对照组心室中 ｃ－ｍｙｃ的表达则与相应心房中 ｃ－ｍｙｃ的表达无显著性差异（ｐ＞ ０．０５）。研究结果提示，训练后心室中ｃ－ｍｙｃ表达的显著下降，一方面，可能是由于在耐力训练应激下，ｃ－ｍｙｃ主要为早期瞬间表达，在训练后早期，甚至几小时即快速表达，然后启动心肌结构基因，如ＭＨＣ、ａ－ａｃｔｉｎ、ＭＬＣ－２、ＡＮＦ等的表达，而自身则由于受反馈抑制的影响，后期表达下降；另一方面，可能由于训练大鼠心房中ｃ－ｍｙｃ表达的触发机制不同于心室中相应机制的缘故。
In order to approach the developing mechanism of cardiac remodeling induced by exercise, the expression of cardiac Proto-oncongen c-myc in 12 week-endurance trained rats was investigated by quantitative reverse transcription polymerase chain reaction technique (QRT-PCR), using the 18S-RNA of rat as a internal reference.The results showed that the expression of ventricular c-myc in endurance trained rats not Only did not increase, but also significantly decrease 17% as compared with their controls (P < 0.001 ). The expression of atrium c-myc in endurance trained rats rats increased 61% as compared with their controls, but there was not statistics significance (P>0. 05 ). The expression of ventricular c- myc in endurance trained rats were significantly decreased expression of ventricular c-myc in endurance trained rats, on the one hand, was that the expression of c-myc was rather early and transient, generally, a enhanced expression of c-myc appeared in several hours after induction, then drived the expression of myocardial structural genes, such as MHC.α-actin.MLC-2 and ANF etc. while its own later expression could be de- creased by the feedback inhibition of secontary genes; on the other hand, it was also possible that the triggering mechanism of c-myc expression in atrium might be different from that in ventricle.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
1999年第2期111-114,共4页
Chinese Journal of Sports Medicine
