摘要
目的：探讨Ｐ物质（ＳＰ）对嗜酸性粒细胞（Ｅｏｓ）粘附功能的调节作用及机制．方法：用收集５１Ｃｒ标记的白细胞分析法观察人Ｅｏｓ对培养的人脐带静脉血管内皮细胞（ＨＵＶＥＣ）的粘附作用．在实验中以不同浓度ＳＰ和ＳＰ＋血小板激活因子（ＰＡＦ）分别预处理Ｅｏｓ，观察其对经ＩＬ－１β活化的ＨＵＶＥＣ粘附性变化．以ＧＲ７１２５１（ＮＫ－１受体阻断剂）和抗ＣＤ１１ｂ作为干预因素，观察它们对ＳＰ＋ＰＡＦ作用的影响．此外，我们还以ＳＰ预刺激ＨＵＶＥＣ，观察其对经ＳＰ＋ＰＡＦ活化人Ｅｏｓ的粘附性变化．结果：单纯ＳＰ不能增加Ｅｏｓ对经ＩＬ－１β活化的ＨＵ－ＶＥＣ的粘附性，却能显著增加ＰＡＦ的促粘效应，这一作用呈剂量依赖性并且被ＧＲ７１２５１和抗ＣＤ１１ｂ所阻断，阻断率分别为２９．２％和５９．２％．ＳＰ预处理ＨＵＶＥＣ后，ＨＵＶＥＣ对ＳＰ＋ＰＡＦ活化的Ｅｏｓ的粘附性增强，也呈剂量依赖性，ＧＲ７１２５１对此有显著的抑制作用．结论：ＳＰ能促进ＰＡＦ对人Ｅｏｓ的粘附性，ＮＫ－１受体和ＣＤ１１ｂ可能参与这一过程；ＳＰ还能激活ＨＵＶＥＣ，使其对Ｅｏｓ的粘附作用增强．
AIM: To investigate the effects of substance P(SP) on the human eosinophil adhesion to endothelial cells and its mechanism. METHODS: Eosinophil adherence to human umbilical vein endothelial cells (HUVEC) was measured by using a 51Crradiolabeled leukocyte collection assay. In the experiment, we observed the influence of SP, combination of SP and platelet activating factor (PAF) on the human eosinophil adherence to HUVEC, respectively. Additionally,we observed whether GR71251, NK1 receptor specific antagonist, and antiCD11b would influence the effect of SP. Adhesion of SPtreated HUVEC to human eosinophil activated by SP and PAF was also observed in this study. RESULTS: SP did not increase the adhesion of human eosinophil to the HUVEC activated with IL1B alone but could significantly enhance the upregulative effect of PAF on adhesion of human eosinophil to HUVEC activated by IL1B.GR71251 and antiCD11b could inhibite the effect of SP respectively (inhibition rate: 29.2% and 59.2%, respectively). The HUVEC activated by SP showed increased adhesion to the eosinophie activated by SP and PAF. The effect of SP on adhesion of HUVEC to eosinophil was dosedependent,and inhibited by GR71251. CONCLUSION: SP can enhance the upregulative effect of PAF on the eosinophil adherence to HUVEC. NK1 receptor and CD11b may be involved in the effect of SP. SP activates the HUVEC, and increases the adhesion of HUVEC to eosinophil activated by SP and PAF.
出处
《第四军医大学学报》
1999年第4期319-322,共4页
Journal of the Fourth Military Medical University
