摘要
应用SYBR Green I染料能选择性结合双链DNA的特点,可检测到沙门氏菌fimI基因特异性靶序列扩增所产生的荧光信号,通过熔解曲线可知其熔点值约为85.6℃,而对其他非沙门氏菌则检测不到荧光信号。建立了一种肉品中的沙门氏菌Real-time PCR检测方法,用该方法检测市售牛肉、香肠中的沙门氏菌,其检测灵敏度分别为13,12 cfu/25 g,从样品的处理到得出检验结果可以在10 h内完成。该检测方法具有简便、快速、特异性强、敏感度高等特点。
As SYBR Green Ⅰ could selectively bind to double-stand DNA,it is possible to identify the fimI gene in Salmonella through fluorescence and to confirm the melting point of about 85.6 ℃ through the melting curve,while other non-Salmonella had no fluorescence.And Real-time PCR assay was developed for the detection of Salmonella in meat products.The detection limits of Real-time PCR assay were 13 cfu/25 g beef and 12 cfu/25 g sausage.The entire detection of Salmonella for samples could be completed within 10 hours or even less from the enrichment to the showing the results.It is concluded that PCR is a specific,sensitive,rapid,and convenient method for detection of Salmonella.
出处
《山西农业科学》
2010年第8期71-76,共6页
Journal of Shanxi Agricultural Sciences
基金
山西省科技攻关项目(2006031066-2)
山西高校科技研究开发项目(20051219)
