摘要
目的:利用HPLC-CL在线检测消癌平注射液(通关藤)对H2O2和O2·-自由基的清除活性,追踪其物质基础。方法:消癌平注射经HPLC后进入T型混合管(内含鲁米诺+过氧化氢溶液和鲁米诺+连苯三酚),记录化学发光信号结合LC-MS/MS鉴定其结构。结果:已鉴定出3-咖啡酰奎尼酸、5-咖啡酰奎尼酸和4-咖啡酰奎尼酸3种物质,清除自由基能力较强。结论:消癌平注射液中酚酸类成分对其清除H2O2和O2·-自由基有很大贡献;HPLC-CL在线联用技术简单、快速,可用于中药抗氧化活性成分的筛选。
AIM： To study the scavenging activities of Xiaoaiping Injection （Marsdenia tenacissima （Roxb） wight et Arn） for H2O2 and O2·- radicals by HPLC coupled with chemiluminescence and to trace the active constituents on line. METHODS： Solution of Xiaoaipin Injection was passed through HPLC to enter into the mixing tees （contained the luminol hydrogen peroxide solution and luminol pyrogallol solution,respectively）,and recorded chemiluminescence signals were recorded,and their chemical structures were identified in combination with LCMS/MS. RESULTS： Three phenolic acids were identified,3-Caffeoylquinic acid,5-Caffeoylquinic acid,4-Caffeoylquinic acid,having scavenging activities for free radicals. CONCLUSION： Phenolic acids in Xiaoaiping Injection make a significant contribution to its scavenging activities for H2O2 and O2·-. It is shown that the method for on-line detection is an advantageous tool to rapidly determine antioxidants from traditional Chinese medicine.
出处
《中成药》
CAS
CSCD
北大核心
2010年第8期1386-1390,共5页
Chinese Traditional Patent Medicine
