摘要
目的观察高迁移率族蛋白B1(HMGB1)对人牙周膜成纤维细胞表达白细胞介素-6(IL-6)、破骨细胞核因子κB受体活化因子(RANKL)、骨保护因子(OPG)的影响,初步探讨HMGB1在牙周疾病的作用。方法采用原代组织块培养法,培养人牙周膜成纤维细胞,用第4～6代的细胞进行实验。分别用10、30、100ng·mL-1质量浓度的HMGB1孵育牙周膜成纤维细胞24h后,RT-PCR检测IL-6、RANKL、OPG的mRNA表达;Westernblot法检测RANKL、OPG的蛋白表达。均以0ng·mL-1质量浓度组为对照,所得数据用单因素方差分析处理。结果 HMGB1在10、30、100ng·mL-1质量浓度时,细胞中的RANKL/OPGmRNA的比值增高(P<0.05),100ng·mL-1质量浓度时细胞中的IL-6mRNA的表达量增高(P<0.05)。Westernblot检测结果显示10ng·mL-1质量浓度组的RANKL/OPG的比值有明显增高。结论一定浓度的HMGB1可使牙周膜细胞中的RANKL/OPG比值增高,还会诱导炎症因子IL-6mRNA表达上调。提示HMGB1可能会在牙周炎的发病以及炎症进展中发挥作用。
Objective To investigate the influence of high mobility group box 1（HMGB1） on the expression of interleukin 6（IL-6）,receptor activator of nuclear factor-kappa B ligand（RANKL） and osteoprotegerin（OPG） on periodontal ligament fibroblasts.Methods Human periodontal ligament fibroblasts were stimulated with HMGB1 at concentrations of 10,30,and 100 ng·mL^-1 for 24 h.RT-PCR and Western blot analysis were performed to check mRNA and protein expression of IL-6,RANKL and OPG on the cells.Results The ratio of RANKL/OPG was increased at both mRNA and protein level after HMGB1 stimulation at 10,30,100 ng·mL^-1.Inflammatory cytokine IL-6 was upregulated by HMGB1 at the concentration of 100 ng·mL^-1.Conclusion Increased ratio of RANKL/OPG and IL-6 on periodontal ligament fibroblasts suggests that HMGB1 might play a role in the pathogenesis and progression of periodontal disease.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2010年第4期443-446,共4页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(30772425)
山东省科技攻关基金资助项目(2009GG10002052)
