摘要
目的:探讨大鼠肢体缺血再灌注损伤(IRI)后脊髓神经元fos、热休克蛋白70(HSP70)的表达改变及丹参注射液对其的影响。方法:通过暂阻断一侧髂总动脉和股动脉,IRI模型。采用免疫组织化学ABC法观察大鼠脊髓神经元fos和HSP70阳性神经元的表达和分布特点,以及丹参注射液干预后阳性神经元改变情况。结果:大鼠肢体缺血4h,脊髓相应节段神经元Fos、HSP70表达增强(P<0.01),再灌注2h后,Fos、HSP70表达进一步增强(P<0.05),并且Fos、HSP70阳性神经元较集中分布于脊髓相应节段同侧后角及中央管周围(P<0.01)。而丹参注射液可进一步增强HSP70表达(P<0.01),并有抑制Fos表达(P<0.01)。结论:脊髓相应节段同侧神经元可能参与肢体IRI的发病过程,尤其是脊髓后角及中央管周围神经元可能具有更为重要的作用。丹参注射液可能对肢体IRI后脊髓神经元有保护作用,这种保护作用可能与其增强HSP70表达和抑制Fos表达有关。
Objective：To investigate spinal cord neurons fos,heat shock protein 70（HSP70）change and influence of Danshen Injection after the rats’limb ischemia reperfusion.Methods：Through temporarily interdict the rat’s one side iliac artery and femoral artery to establish the model of the limb ischemia reperfusion injury.Using the immunohistochemical method of ABC to observe the expressions about neurons fos and HSP70 positive neurons and the character about their distributions,as well as their change after being intervened by Danshen Injection n.Results：The rats’spinal cord neurons fos and HSP70 expressions increased in four hours later the limb ischemia（P0.01）,after reperfusion for 2h,neurons fos and HSP70 expressions further enhanced（P0.05）.And the positive neurons Fos and HSP70 distributions were concentrated in the spinal cord with the corresponding section and around the central tube（P0.01）.Danshen injection can enhance HSP70’s expressions（P0.01）,and inhibit the effect of neurons fos（P0.01）.Conclusion：The corresponding section of spinal cord neurons may participate in the process of limb IRI,especially the cornu posterius medullae spinalis and the around central tube’s neurons may have a more important role.Danshen Injection was likely to protect the spinal cord neurons after limb IRI,the protection may relative to the expression of HSP70 enhance and neurons fos inhibit.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2010年第9期1377-1380,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.30300461
No.30973787
No.30973809)~~
