摘要
本试验采用了内蒙古呼和浩特健康荷斯坦奶牛乳腺组织经5代纯化后，将奶牛乳腺上皮细胞分别设为1组（1×10^4个细胞／ml）、2组（1×10^5个细胞／ml）、3组（1×10^6个细胞脚）、4组（1×10^7个细胞／ml）4个密度梯度进行冻存，复苏后检测细胞死亡率、贴壁率、凋亡率并进行形态学观察。结果显示4纽复苏后死亡率极显著低于其他三组（P〈0．01），贴壁率显著高于其他三组（P〈0．05）。3组细胞凋亡率显著低于其他三组（P〈0．05）。3、4组细胞到第3d基本铺满瓶底，生长状态良好。
In this study, the breast tissue of a healthy Holstein dairy cow was used. Through five-generation purification, the bovine mammary epithelial cells were divided into four groups, according to the cell density （1× 10^4 cells/ml, group 1; 1× 10^5 cells/ml, group 2; 1×10^6cells/ml, group 3; 1× 10^7 cells/ml, group 4 respectively） and cryopreservated, then thawed after 1 month and maintained separately. The results showed that group 4 had the lowest death rate, significantly lower to other three treatments （P〈0.01）, and the highest adhesion rate, significantly higher to other three treatments （P〈0.05）. Group 3 had the lowest apoptosis rate, significantly lower to other three treatments （P〈0.05）. The cells of group 3 and group 4 basically covered with the bottom of the culture flask and the cells were in good condition at three days after adherence.
出处
《中国细胞生物学学报》
CAS
CSCD
2010年第4期626-629,共4页
Chinese Journal of Cell Biology
基金
农业部(No.Nycytx-02-05)资助项目~~
关键词
奶牛乳腺上皮细胞
死亡率
贴壁率
凋亡率
bovine mammary epithelial cells
death rate
adhesion rate
apoptosis rate
