摘要
目的研究ｍｄｒ１基因在肾癌组织中的表达状况。方法应用逆转录聚合酶链反应（ＲＴＰＣＲ）技术检测２０例肾癌和５例正常肾组织ｍｄｒ１基因ｍＲＮＡ的表达。结果８５％（１７／２０）的肾癌和１００％（５／５）的正常肾组织表达ｍｄｒ１ｍＲＮＡ。结论肾癌ｍｄｒ１基因的高表达是肾癌耐药的重要原因，检测ｍｄｒ１基因表达可为临床化疗药物的选择提供依据。
PURPOSE To study the expression of mdr 1 gene in renal cell carcinomas(RCC). METHODS The reverse transcription polymerase chain reaction(RT PCR) was used to detect expression of mdr 1 mRNA in 20 RCC and 5 normal kidneys. RESULTS The mdr 1 mRNA was expressed in 85%(17/20) of RCC and all the 5 normal kidneys. CONCLUSIONS The high expression of mdr 1 gene in RCC might be an important factor of its drug resistance.Detection of mdr 1 gene expression in RCC could provide a basis for clinical selection of chemotherapeutic drugs.
出处
《中国癌症杂志》
CAS
CSCD
1999年第2期105-106,共2页
China Oncology
关键词
肾肿瘤
基因
MDR-1
RT-PCR
Kidney neoplasms Gene,mdr 1 Reverse transcription polymerase chain reaction
