摘要
目的了解马鞍山地区不同人群中戊型肝炎病毒(HEV)流行株基因型的分布状况。方法对来自一般人群、有偿献血人群、吸毒人群的血清标本进行HEV IgG、IgM抗体检测并将HEV IgM阳性的血清标本进行RT-PCR扩增,PCR阳性标本进行基因测序及序列分析。结果 HEV PCR扩增成功16份,其中HEV IgM抗体阳性病人和正常人群中10份标本阳性,吸毒人群和有偿献血人群中各3份标本PCR阳性,测序结果显示均为HEVⅣ型毒株感染。结论马鞍山地区不同人群中HEV流行株均为基因Ⅳ型,但不同人群内部的流行株存在较大变异。
Objective To analyze genotype distribution of hepatitis E virus（HEV） isolated from different populations of Maanshan.Methods HEV antibody was detected by using enzyme immunoassay for HEV positive samples.RT-PCR based on universal primers for multiple HEV genotypes was used to detect HEV RNA in the serum samples.PCR products were sequenced and analyzed with the DNASTAR and MEGA3.1 softwares.Results 16 out of the 53 samples were positive for RT-nPCR.All of the isolates were clustered into HEV genotype Ⅳ.The nucleotide sequences of the HEV isolates of genotype Ⅳ presented high variability in former commercial blood donations.Conclusions The data from this study suggested that the isolates from different population were HEV genotype Ⅳ and the genetic variability is high.
出处
《中华疾病控制杂志》
CAS
2010年第7期613-615,共3页
Chinese Journal of Disease Control & Prevention
基金
安徽省高校自然科学基金(KJ2008B89ZC)
马鞍山市科技计划项目(2008-40)
关键词
肝炎病毒
戊型
基因型
序列分析
逆转录聚合酶链反应
Hepatitis E virus
Genotype
Sequence analysis
Reverse transcriptase polymerase chain reaction
