摘要
目的探讨p53基因和hMLH1基因的突变是否是直肠癌发生的早期事件之一。方法用PV-9000二步法免疫组化检测技术对我院2003年2月至2009年7月期间的32例早期直肠癌、32例直肠腺瘤及30例正常直肠黏膜组织石蜡切片进行p53和hMLH1基因蛋白表达的检测。结果①正常直肠黏膜组织、直肠腺瘤组织及早期直肠癌组织中p53蛋白表达阳性率分别为0(0/30)、59.38%(19/32)及68.75%(22/32),其在直肠腺瘤组织和早期直肠癌组织中的p53蛋白表达阳性率差异无统计学意义(P>0.05),但二者均明显高于其在正常直肠黏膜组织中的表达(P<0.01);hMLH1蛋白在前述三种组织中的表达阴性率分别为0(30/30)、12.50%(4/32)和50.00%(16/32),其在早期直肠癌组织中的表达阴性率明显高于在直肠腺瘤组织和正常直肠黏膜组织中的表达(P<0.01)。②在直肠腺瘤组织和早期直肠癌组织中p53阳性表达同时合并hMLH1阴性表达者分别占9.38%(3/32)及37.50%(12/32),二者间差异有统计学意义(P=0.008)。③p53阳性表达和hMLH1阴性表达均与早期直肠癌的分化程度有关(P<0.05),而均与患者的性别、年龄、肿瘤最大径、浸润深度及大便潜血均无关(P>0.05)。④p53阳性表达与腺瘤的增生程度有关(P=0.009),hMLH1阴性表达与腺瘤的增生程度无关(P>0.05)。结论 p53基因和hMLH1基因的同时突变致癌作用明显,可能是直肠癌发生的早期事件之一。
Objective To explore whether mutations of p53 gene and hMLH1 gene may be an early event of carcinogenesis in rectal cancer. Methods The expressions of p53 and hMLH1 protein in 32 patients with early rectal cancer,32 patients with rectal adenoma,and 30 patients with normal rectal mucosa were detected by PV-9000 immunohistochemical method between February 2003 and July 2009 in this hospital. Results ① The positive expression rates of p53 protein were 0 （0/30）,59.38% （19/32）,and 68.75% （22/32） in the normal rectal mucosa,rectal adenoma,and early rectal cancer,respectively. There was no difference between the rectal adenoma and early rectal cancer （P0.05）,but which were higer than that of the normal rectal mucosa （P0.01）. The negative expression rates of hMLH1 protein were 0 （0/30）,12.50% （4/32）,and 50.00% （16/32） in the normal rectal mucosa,rectal adenoma,and early rectal cancer,respectively. The negative expression rate of hMLH1 protein in the early rectal cancer was significantly higher than that in the rectal adenoma or the normal rectal mucosa （P0.01）. ② The positive expression of p53 concomitant negative expression of hMLH1 in the rectal adenoma and early rectal cancer were 9.38% （3/32） and 37.50% （12/32）,respectively,the difference was statistically significant （P=0.008）. ③ In the early rectal cancer,the positive expression of p53 and the negative expression of hMLH1 were closely related to the degree of differentiation （P0.05）,but which weren＇t related to the patient＇s gender,age,tumor maximum diameter,depth of invasion or fecal occult blood （P0.05）. ④ The positive expression of p53 was closely related to higher adenoma hyperplasia （P=0.009）,while not of negative expression of hMLH1 （P0.05）. Conclusion Simultaneous mutations of p53 gene and hMLH1 gene may be an early event of carcinogenesis in rectal cancer.
出处
《中国普外基础与临床杂志》
CAS
2010年第9期889-893,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
民政部十一五老年医学科研项目(项目编号:47-2-17)~~
