摘要
建立了牛奶和奶粉中三聚氰酸(CYA)的高效液相色谱-串联质谱(HPLC-MS/MS)测定方法。样品用乙腈提取并沉淀蛋白,经强阴离子交换柱富集和净化,AX色谱柱分离,HPLC-MS/MS法测定,内标法定量。50～2000μg/L范围内CYA的线性关系良好(r≥0.999);在奶粉和牛奶基质中,添加200、500和1000μg/kg3个添加水平的回收率均在97%～121%之间,相对标准偏差(RSD)均小于4.8%;定量限(LOQ)为200μg/kg。方法的前处理快速简便,净化效果好,准确度和精密度高,可用于牛奶和奶粉中CYA的测定。
A method for the determination of cyanuric acid（CYA） in milk and milk powder was developed using high performance liquid chromatography-electrospray ionization tandem mass spectrometry（HPLC-ESI MS/MS）.The target analyte was extracted from samples by acetonitrile,and the proteins were precipitated at the same time.The supernatant was cleaned up and enriched with a strong anion exchange column,analysed by HPLC-MS/MS with an AX column,and quantified by internal standard method.Good linearity was obtained in the range of 50-2000 μg/L.The recoveries were all ranged from 97% to 121% at three levels,200,500 and 1000 μg/kg,and the relative standard deviations（RSDs） were all below 4.8%.Limit of quantification（LOQ） was 200 μg/kg.The method has high accuracy and precision,the operation is fast,easy and suitable for the determination of CYA in milk and milk powder.
出处
《色谱》
CAS
CSCD
北大核心
2010年第9期859-862,共4页
Chinese Journal of Chromatography
关键词
高效液相色谱-串联质谱
三聚氰酸
牛奶
奶粉
high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）
cyanuric acid
milk
milk powder
