摘要
目的探讨我市孕前和孕期女性叶酸利用能力和不良孕产史的关系,从而为我市妇女提供孕期叶酸补充和孕期风险监测指导,提高出生人口素质。方法通过检测叶酸代谢过程中重要酶的编码基因5,10-亚甲基四氢叶酸还原酶(MTHFR)和甲硫氨酸合成酶还原酶(MTSR)的相关多态性位点,对入组的孕期女性的叶酸代谢能力进行分级。结果三亚女性中,叶酸代谢最重要的遗传危险因素MTHFR 677TT的频率低于全国水平近15%,而在风险基因型MTHFR 1298CC和MTSR 66GG的比例高4%左右。不良孕产者中高、中、低及未发现风险人群的比例分别为31.07%、38.83%、5.83%和24.27%,而正常孕产者中分别为19.45%、38.23%、4.20%和38.12%,两组人群在叶酸利用能力风险等级分类上存在差异(P<0.05)。结论叶酸代谢和利用障碍与不良孕产有着重要的关系。
Aim To investigate the correlation of capacity of pregnant and pying-in women in utilization of folate with unhealthy gestational and delivering history. Methods The relevant polymorphism sites of 5,10-methylenetetra-hydrofolate reductase （MTHFR）gene and methionine synthase reduetase （MTSR）gene were determined and anlayzed by fluorescence quantitative polymerase chain reaction（FQ_PCR）. Results The frequency of MTHFR677TY THE most risk factor related to metabolism and utilization of folate was 15% lower as compared to that of the national level and the frequency of another risk gentype MTHFR1298CC and MTSR 66GG was 4% higher than tht of the national level. The rates of high,moderate,low and without risk persons were 31.07% ,38.83%,5.83% and 24.2%. While that in the normal pregnant and lying-in women were 19.45% ,38.23%,4.20% and 38.12%.,Showing significant differences in risk grade of utilization of folate between two groups. Conclusion The disorder in metabolic and utiliztion of folate is associated with unheathy gestational and delivering history.
出处
《中国热带医学》
CAS
2010年第11期1346-1347,1351,共3页
China Tropical Medicine
基金
国家高技术研究发展计划(863计划)(编号2006AA02Z433)
关键词
孕产妇
叶酸
基因型
荧光定量PCR
Pregnant and lying-in women with unhealthy gestational and dehvering hisotry
Folate
Genotype
Fluorescence quantitative polymerase chain reaction（FQ-PCR）
