摘要
目的研究视网膜光化学损伤中前列腺素（ｐｒｏｓｔａｇｌａｎｄｉｎ，ＰＧ）含量变化以及地塞米松（ｄｅｘａｍｅｔｈａｓｏｎｅ，ＤＸＭ）对这种变化的影响。方法３６只ＳＤ大鼠分为对照组和ＤＸＭ组，后者于光照前３天开始，每日腹膜腔注射ＤＸＭ（１ｍｇ／ｋｇ），连续５天。两组大鼠经（１９００±１０６．９）ｌｘ，波长为５１０～５６０ｎｍ的光连续照射２４小时，于光照前及光照结束后的６小时和１，３，７，１４天分别摘除眼球，用放射免疫分析法进行视网膜前列腺素Ｅ２（ｐｒｏｓｔａｇｌａｎｄｉｎＥ２，ＰＧＥ２）与６－酮－前列腺素Ｆ１α（６－ｋｅｔｏ－ＰＧＦ１α）含量检测。结果对照组光照后６小时，１，３天ＰＧＥ２的含量为（３７．５０±２．７５，４８．０６±４．０４，８１．９０±４．８９）ｐｇ／ｍｇ，６－ｋｅｔｏ－ＰＧＦ１α的含量为（４．６８±０．６９，７．５０±０．５７，１０．４０±０．７１）ｐｇ／ｍｇ，明显高于ＤＸＭ组相应时间点的ＰＧＥ２（２０．６０±４．２８，３７．３６±３．３４，５４．８５±４．５７）ｐｇ／ｍｇ及６－ｋｅｔｏ－ＰＧＦ１α（２．５０±０．５９，４．６８±０．８１，６．８７±１．１０）ｐｇ／ｍｇ（Ｐ＜０．０１）。结论ＤＸ?
Purpose To evaluate the prostaglandins(PG) levels
and to identify the effect of dexamethasone(DXM) on PG in response to photochemical insult in
rat retina. Methods The experiments were performed on 36 SD rats which were separated into
two groups,control and treated groups,and the latter received daily intraperitoneal injections of
DXM (1mg/kg)for 5consecutive days,starting 3 days before light exposure.The animals were
continually exposed to green fluorescent light(510560 nm)with an illuminance level of (1900106.
9)lx for 24 hrs.The retinal concentration of PGE2 and 6ketoPGF1
weretestedat6hrs,1,3,7and14daysafterlightexposure. Results ThePGE2and6ketoPGF1levels of
the control groups (37.502.75,48.064.04,81.904.89) pg/mg and
(4.680.69,7.500.57,10.400.71)pg/mghad significantly higher values than those of the treated
rats(20.604.28,37.363.34,54.854.57)pg/mg and (2.500.59,4.680.81,6.871.10)pg/mg( P <0.01)after
6hrs,1and 3 days light exposure respectively. Conclusion By inhibition of PG
synthesis,the DXM may play an ameliorative effect on retinal photochemical injury of rats.
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
1999年第2期94-96,共3页
Chinese Journal of Ocular Fundus Diseases
基金
国家自然科学基金
关键词
视网膜
病理学
前列腺素
光化学损伤
Retina/pathologyPhoticstimulationProstaglandins/analysisDexamethasone/pharmacologyDisea
se models,animal
