摘要
目的 99Tc-MDP治疗骨质疏松动物模型,探讨与骨生物力学的关系。方法实验分二阶段,第1阶段,采用每周2次肌肉注射3mg/kg盐酸地塞米松量的方法,连续6周,建立骨质疏松模型。第2阶段,在兔骨质疏松模型的基础上,分别设立99Tc-MDP、"帕米磷酸二钠"和"氨基二膦酸盐"治疗组,并且与正常对照组和骨质疏松动物模型对照组进行比较。各组动物治疗参照成人的用量及用法,治疗时间16周。试验结束后,进行X线摄片、CT摄片、核素骨显像半定量测定、骨密度测定、骨生物力学测定和血清BALP、BGP检测。结果第1阶段,正常组病理细胞学显示,骨小梁排列规则,呈正常骨组织形态,而骨质疏松组则为骨小梁排列稀疏,存在较明显的骨小梁断裂现象,稀疏和断裂的骨小梁间存在脂肪组织填充,骨组织呈破坏形态,核素骨骼显像半定量测定、血清BALP、BGP检测增高,骨密度测定、骨生物力学测定降低,二组间存在较明显的差异,各项指标存在统计学意义(P<0.01),证实骨质疏松动物模型成立。第2阶段,在骨质疏松模型的基础上,经99Tc-MDP、"帕米磷酸二钠"和"氨基二膦酸盐"治疗组治疗后,在病理细胞学、核素骨骼半定量显像、骨密度测定、骨生物力学测定和血清BALP、BGP检测都存在不同的改善,而99Tc-MDP治疗组的数据改善明显,各组间存在统计学意义(P<0.01)。结论 99Tc-MDP治疗的兔骨质疏松模型在骨生物力学的较明显增强变化与骨细胞学、骨形态计量、骨密度、骨显像、血清BALP、BGP的变化相匹配,说明亚甲基二膦酸钠经螯合微量元素99Tc后,存在可降低骨骼脆性的价值。
Objective Using 99Tc-MDP osteoporosis animal treatment model to investigate its influence to the bone biomechanics. Methods On stage 1, rabbits were used to establish osteoporosis animal model by injecting dexamethasone intramuscularly, 3 mg/kg twice a week for 6 weeks. On stage 2, based on the established rabbit osteoporosis model, the animals were divided into 99Tc-MDP treatment group, pamidronate treatment group, and alendronate sodium treatment group. The treatment groups were compared to the normal control group and the osteoporosis animal model group. All dosages used were according to human adult dosages and continued for 16 weeks in the treatment groups. At the end of the study, X-ray scan, CT scan, semi-quantitative nuclear bone scan, bone cell biology, bone density, bone biomechanical parameters, serum BALP, and serum BGP were examined. Results On the stage 1, bone skeleton framework showed normal structure in the normal control group. However, in the osteoporosis group, bone skeleton framework showed obvious loose construction and bone fracture. Fat tissue existed between the loosen and fractured bones. The structure of the bone tissue was destroyed. The values of semi-quantitative nuclear bone scan, BALP, and BGP elevated. The values of bone density and bone biomechanical parameters decreased. The difference between the two groups was statistically significant （ P 〈 0.01 ）. Therefore the osteoporosis animal model was established. On the stage 2, based on the osteoporosis model, parameters of bone cellular pathology, semi-quantitative nuclear bone scan, bone cell biology, bone density, bone biomechanics, serum BALP, and serum BGP were improved in 99 Tc-MDP treatment group, pamidronate treatment group, and alendronate sodium treatment group. The best result appeared in the 99 Tc-MDP treatment group. Comparisons among groups showed statistical differences（ P 〈 0.01 ）. Conclusion 99Tc-MDP showed that its increased bone biomechanical effect was comparable to its effect in bone cytology, bone histomorphometry, bone density, bone scintigraphy, model. This demonstrated that bisphosphonates can serum BALP, and serum BGP in the osteoporosis rabbit decrease bone fragility by coordinating with 99Tc.
出处
《中国骨质疏松杂志》
CAS
CSCD
2010年第9期640-643,646,共5页
Chinese Journal of Osteoporosis
