摘要
目的：探讨玄参化学成分和药理活性关系。方法：应用大孔树脂、葡聚糖凝胶和正、反相硅胶等层析方法，摸索多种溶剂系统对玄参中含量高的水溶性成分进行分离，应用化学和光谱方法鉴定结构。观察分离得到的主要单体成分对大鼠腹腔中性白细胞中花生四烯酸（ＡＡ）代谢产物白三烯Ｂ４（ＬＴＢ４）的影响，及对阈剂量的二磷酸腺苷，ＡＡ和２０ｎｍｏｌ／ＬＰＡＦ诱导的血小板聚集的抑制作用。结果：共分得１１种玄参水溶性成分，发现它们属于环烯醚萜甙和苯丙素甙类型。药理实验中苯丙素甙ＸＳ－８，ＸＳ－１０在０．５ｍｍｏｌ／Ｌ时对ＬＴＢ４产生较强抑制作用；环烯醚萜甙ＸＳ－６，ＸＳ－７在相同条件下作用较弱。苯丙素甙和环烯醚萜甙在０．５ｍｍｏｌ／Ｌ时，对体外诱导的血小板聚集都有不同程度的作用，但苯丙素甙的作用强于环烯醚萜甙。结论：玄参中的苯丙素甙和环烯醚萜甙成分与玄参的抗炎和抗血小板聚集作用有关。
Objective: To investigate
the relationship between constituents and pharmacological effects of Scrophularia ningpoensis
(XuanSheng). Methods: The isolation and identification of compounds were done mainly by
nonpolar macroreticular resin, sephadex LH20, ODS, silica gel column chromatography and
FABMS, NMR etc, spectra. Results: The inhibition of the LTB4 formation in the rat abdominal
neutral leukocytes by drugs isolated from S. ningpoensis were observed. The iridoid
glycosides XS6, XS7 showed weak action, but the phenylpropanoid glycosides XS8, XS10
showed potent action at the concentration of 0.5 mmol/L. In the inhibition of the rabbit platelet
aggregation, iridoid and phenylpropanoid glycosides were active in inhibiting the rabbit
threshold ADPinduced and AAinduced but not 20 nmol/L PAFinduced platelet aggregation at the
concentration of 0.5 mmol/L in vitro. Conclusion: The phenylpropanoid and iridoid glycosides
may be associated with antiinflammation and antiplatelet aggregation effects of S. ning
poensis.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第5期301-303,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
关键词
玄参
环烯醚萜甙
苯丙素甙
血小板聚集
LTB4
Scrophularia ningpoensis
iridoid glycoside
phenylpropanoid glycoside
leukotrienes B
platelet aggregation
