摘要
背景:脐带血干细胞富含多种干/祖细胞,其各种干/祖细胞移植治疗和作为种子细胞在组织工程领域的研究成为当前的热点。目的:综述脐带血干细胞中存在的多种干/祖细胞的生物学特性及在心脏疾病的实验应用和脐带血干细胞在心脏疾病的实验应用。方法:应用计算机检索1999-01/2009-10中国数字图书馆、维普期刊数据库相关文章,检索词为"脐带血干细胞,造血干/祖细胞,间充质干细胞,内皮祖细胞,内皮细胞,心肌细胞,组织工程,种子细胞",并限定文章语言种类为中文。同时计算机检索1999-01/2009-10PubMed数据库相关文章,检索词为"umbilical cord blood stem cells,hematopoietic progenitor cells/hematopoietic stem cells,mesenchymal stem cells,endothelial progenitor cells,endothelial cells,myocardial cells,tissue engineering,seeded cells",并限定文章语言种类为English。同时手工检索《干细胞生物学》专著,共检索到文献95篇。根据纳入标准最终纳入文献30篇。结果与结论:脐带血干细胞是一种成体多能干细胞,富含造血干/祖细胞、间充质干细胞、内皮祖细胞、非限制性体干细胞等。相对于骨髓干细胞和外周血干细胞,脐带血干细胞来源丰富,获得更为容易,更为原始,分化增殖能力更强,排斥反应小,在体外可以分化为血管内皮细胞、心肌细胞等多种细胞。基于脐带血干细胞独特的生物特性、资源优势及在冠心病和缺血性心肌病的治疗上取得了一定的成果,为构建组织工程人工心脏的种子细胞来源提供可能,在心脏再生和组织工程人工心脏方面的应用展示了广阔的前景。
BACKGROUND：Umbilical cord blood stem cells contain a variety of stem/progenitor cells. Usage of these stem/progenitor cells for transplantation therapy and as seed cells in tissue engineering research is a current research focus. OBJECTIVE：To review the biological characteristics of various types of stem /progenitor cells contained in umbilical cord blood stem cells and related stem cell-based experimental application for treatment of heart diseases. METHODS：To search related literatures by computer retrieval in China Hospital Knowledge Database and Chongqing VIP information web by using key words as ＂umbilical cord blood stem cell,hematopoietic progenitor/ stem cells,mesenchymal stem cells,endothelial progenitor cells,endothelial cells,myocardial cells,tissue engineering,seed cells＂ in Chinese from January 1999 to October 2009. Further literatures searching was tried in PubMed database with search terms limited to ＂umbilical cord blood stem cell,hematopoietic progenitor cells/hematopoietic stem cells,mesenchymal stem cells,endothelial progenitor cells,endothelial cells,myocardial cells,tissue engineering,seed cells＂ in English from January 1999 to October 2009. In addition,manual retrieval of the monograph of Stem Cell Biology was also done. A total of 95 documents were retrieved. According to the inclusion criteria,30 documents were finally selected. RESULTS AND CONCLUSION：Umbilical cord blood stem cells are a kind of adult multipotent stem cells,including hematopoietic progenitor cells/hematopoietic stem cells,mesenchymal stem cells,endothelial progenitor cells,unrestricted somatic stem cells and so on. Umbilical cord blood stem cells contain rich primitive stem cells,which have stronger differentiation and proliferation capacity,smaller rejection reaction and get more easily than that of bone marrow stem cells and peripheral blood stem cells. Umbilical cord blood stem cells can differentiate into many kinds of cells such as myocardial cells and vascular endothelial cells in vitro. Based on the unique biological characteristic and source superiority of umbilical cord blood stem cells,the outcome in treatment of coronary heart disease and ischemic cardiomyopathy had obtained. Umbilical cord blood stem cells may be a new source of seed cells for construction tissue engineered artificial heart,and show broad prospects for research of heart regeneration and tissue engineered artificial heart.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第32期6027-6030,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
四川省应用基础研究基金(2008JY0689-1)~~
