摘要
目的:比较健康老年男性和脾虚证老年男性患者肠道菌群结构特征,研究脾虚证与肠道菌群的关系。方法:分别建立老年男性健康和脾虚证患者(脾肾阳虚、脾气虚、脾肾阳虚兼脾气虚)粪便肠道菌群变性梯度凝胶电泳(DGGE)指纹图谱,并进行非加权配对算术平均法聚类分析、多维尺度分析和主成分分析,确定老年脾虚证患者肠道菌群的基因指纹特征。结果:脾肾阳虚、脾气虚和脾肾阳虚兼脾气虚患者的肠道菌群结构具有明显特征,并与临床诊断结果基本一致;同一证型不同病症、临床表征和病程的患者肠道菌群结构不同。结论:粪便肠道菌群16SrDNADGGE图谱分析方法适用于脾虚证患者肠道菌群变化研究,是现代生物技术与传统中医理论相结合的实际应用。
Objective：To investigate the intestinal microflora of healthy subjects and elderly patients with spleen deficiency and reveal the relationship of intestinal microfiora and spleen deficiency.Methods：Human intestinal microflora 16S rDNA fingerprinting was constructed by 16S rDNA DGGE,comparing the difference between healthy subjects and elderly spleen deficiency patients by similarity analyses（including Unweighted Pair Group Method with Arithmetic cluster analysis,Multi-dimension Analysis,Principal Component Analysis）.Results：It was found that the intestinal microflora composition of deficiency of spleen yang,deficiency of spleen qi and deficiency of both spleen qi and spleen yang patients were distinct.Futhermore,the intestinal microflora composition of spleen deficiency subjects varied with different subcategories,symptoms and course of diseases.Conclusions：The 16S rDNA DGGE fingerprint can reveal the changes of intestinal microflora.Our study investigated the intestinal microflora of elderly patients with spleen deficiency,which could contribute to modernization of traditional Chinese medicine.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2010年第10期1566-1569,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
"十一五"国家科技支撑计划重点项目(No.2006BAI08B05-02)~~
