摘要
目的探讨表面活性蛋白D(SP-D)对吞噬细胞吞噬幽门螺杆菌(HP)的影响。方法从SD大鼠肺泡灌洗液中提取并纯化SP-D。将试验组HP11637、HPSS1菌液分别用SP-D预处理30min,加入小鼠腹腔巨噬细胞培养液。将细胞与细菌混合物置于细胞培养箱中37℃分别孵育0、30、60min后,用头孢唑啉钠杀灭细胞外的细菌。裂解巨噬细胞后,取适量细胞裂解稀释液接种于微需氧环境中孵育3d后,计数菌落形成单位。同时设置未经SP-D预处理的HP11637、HPSS1加入小鼠腹腔巨噬细胞培养液中作为对照组。结果小鼠腹腔巨噬细胞与HP11637共同孵育后0、30、60min,试验组细胞内HP11637数量为(123±8)、(411±38)、(343±44)个/100个巨噬细胞,显著多于对照组的(104±14)、(179±10)、(163±10)个/100个巨噬细胞(P<0.01)。小鼠腹腔巨噬细胞与HPSS1共同孵育后0、30、60min,两组细胞内HPSS1数量相仿(P>0.05)。结论 SP-D具有显著增强小鼠腹腔巨噬细胞体外吞噬HP11637的功能,而对小鼠腹腔巨噬细胞体外吞噬HPSS1无显著影响。
Objective To explore the influence of surfactant protein-D（SP-D）on phagocyte phagocytizing Helicobacter pylori.Methods SP-D was extracted and purified from alveolar irrigating solution in SD rats.HP11637 and HPSS1 pretreated with SP-D for 30minutes were added into the culture fluid of mouse peritoneal macrophages.The mixture of macrophages and bacteria was incubated for 0,30 and 60 minutes at 37℃ in 5% CO2.The extracellular bacteria were killed by cefazolin sodium.The macrophages were splited.The lysates were diluted,which was inoculated on Columbia agar plates and incubated in microaerobic atmosphere.Colony-forming units were counted after 3 days.HP11637 and HPSS1 untreated with SP-D were added in the culture fluid of mouse peritoneal macrophages as the controls.Results After the mixture of macrophages and bacteria pretreated with SP-D incubated for 0,30 and 60 minutes,the numbers of HP11637 were（123±8）,（411±38）and（343±44）piece/100 macrophages,respectively,which were significantly greater than（104±14）,（179±10）and（163±10）piece/100 macrophages（P0.01）.But the numbers of HPSS1 at three time points were not significantly different between two groups.Conclusion SP-D can strengthen phagocytosis of mouse peritoneal macrophages in vitro to HP11637,but has not significant influence on HPSS1.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第17期2057-2059,共3页
Jiangsu Medical Journal
基金
贵州省卫生厅科技基金立项项目(GZWKJ2009-1-073)
