摘要
用高压均质机制备的ＮＤ－ＥＤＳ７６二联油乳剂灭活疫苗，经３０００ｒｐｍ，１０ｍｉｎ离心不破乳，３７℃恒温保存３２ｄ以上未破乳，粘度为３～４Ｓｅｃ；用该油乳剂灭活疫苗接种商品蛋鸡，每羽经肌肉注射０．５ｍＬ，接种１４ｄ后即可产生足够保护力的中和抗体，２个月时间抗体可达高峰；接种５０周后，ＮＤＨＩ抗体效价仍维持在８．２ｌｏｇ２，ＥＤＳ７６ＨＩ抗体效价７．０ｌｏｇ２。在４～８℃冰箱中保存，有效期在一年以上，经８０万头份中间试验证明，该疫苗对外来品种鸡和地方品种鸡同样有效，能有效控制ＮＤ和ＥＤＳ７６两种疾病。
The inactivated vaccine in oily adjuvant against ND and EDS 76 was made with high-pressure homogeneous machine.The stabilty of inactivated vaccine was very well. The inactivated vaccine was used to inoculate commercial egg-laying chicken by muscle injecting 0.5mL per hen, and had good immune effect. The HI antibody valence of ND and EDS 76 still kept at 8.21 log2 and 7.01 log2 respectively at 50 weeks after inoculation.
出处
《江西农业学报》
CAS
1999年第2期10-14,共5页
Acta Agriculturae Jiangxi
